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The effects of serial passage of a nucleopolyhedrosis virus through an alternate host system

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Abstract

The multiple-embedded velvetbean caterpillar nucleopolyhedrosis virus (VBC-NPV) ofAnticarsia gemmatalis Hübner was shown to be infectious of a variety of noctuid hosts. The mortality data demonstrated the importance of defining the dosage used in host range analysis. Serial passage of the VBC-NPV through the soybean looper,Pseudoplusia includens, was done to select for host range variants of this NPV. After several passages of the VBC-NPV through this insect the virulence of the progeny virus remained unchanged indicating heterogeneity in the host and not the virus population. However, between the 3rd and 5th serial passage throughPseudoplusia a latent NPV identical to a single-embedded NPV previously associated fromA. gemmatalis was activated. The biological and biochemical characteristics of this isolate demonstrated it to be distinct from the original VBC-NPV. A single passage of this activated virus throughA. gemmatalis resulted in the production of viral progeny having characteristics of the original VBC-NPV. Serial passage of this virus preparation throughP. includens resulted in virus progeny having biological properties associated with both the original VBC-NPV and the activated NPV isolate.

Résumé

Le virus à polyèdres nucléaires à inclusion multiple d'Anticarsia gemmatalis Hübner est infectieux pour différentes espèces de noctuides. Les données sur la mortalité obtenue montrent l'importance de la définition des doses utilisées pour l'étude de la gamme d'hôtes. Des passages en série de ce virus chez la noctuelle du soja,Pseudoplusia includens Walker ont été réalisés en vue de sélectionner des variants du virus adaptés à d'autres hôtes. Après plusieurs passages chezPseudoplusia, la virulence du virus demeure inchangée, ce qui indique une hétérogénéité de la population de l'hôte mais non de celle du virus. Cependant entre le 3e et le 5e passage chezP. includens un virus occulte identique au virus à polyèdre nucléaire à simple inclusion précédemment identifié chezA. gemmatalis, a été activé. Les caractères biologiques et biochimiques de cet isolat montrent qu'il est différent du virus original deA. gemmatalis utilisé pour cette expérimentation. Un seul passage de ce virus activé chezA. gemmatalis aboutit à la production d'un virus ayant les caractéristiques du virus original à inclusions multiples. Des passages en série, chezP. includens d'une préparation à base de ce virus produisent une descendance ayant les propriétés biologiques à la fois du virus original et de l'isolat activé.

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Authors and Affiliations

  1. Dept. Genetics Unicamp c. p. 1170, 13100, Campinas, S. P. Brazii

    O. H. Pavan

  2. Dept. Entomology and Nematology University of Florida, 32611, Gainesville, Florida

    D. G. Boucias

Authors
  1. O. H. Pavan
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  2. D. G. Boucias
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  3. J. C. Pendland
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Additional information

The investigation reported in this paper (No 2408) is in connection with a project of the Florida Agricultural Experiment Station and is published with approval of the Director.

Supported in part by a fellowship from Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP-=78/0505).

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Pavan, O.H., Boucias, D.G. & Pendland, J.C. The effects of serial passage of a nucleopolyhedrosis virus through an alternate host system. Entomophaga 26, 99–108 (1981). https://doi.org/10.1007/BF02371838

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