Pectolytic enzymes activity and pathogenicity ofGaeumannomyces graminis var.tritici and related Phialophora-like fungi

Abstract

Gaeumannmyces graminis var.tritici (Ggt), Phialophora sp. (lobed hyphopodia) andPhialophora graminicola vere grown in a liquid medium with pectin and on autoclaved wheat roots (root media) and the activity of pectolytic enzymes in culture filtrates was measured. Most strains of the fungi exhibited polygalacturonate trans-eliminase activity but no pectin methylesterase activity was detected.Ggt polygalacturonase was found in culture filtrates from all the media used whilePhialophora sp. did not exhibit activity of this enzyme in the unbuffered root media. No polygalacturonase activity was demonstrated forP. graminicola. A correlation was found (r=0.548) betweenin vitro polygalacturonase activity and the pathogenicity ofGgt to wheat seedlings.

This is a preview of subscription content, access via your institution.

References

  1. Ayers W A, Papavizas G C and Diem A F 1966 Polygalacturonate transeliminase and polygalacturonase production byRhizoctonia solani. Phytopathology 56, 1006–1011.

    CAS  Google Scholar 

  2. Barker K R and Walker J C 1962 Relationship of pectolytic and cellulelytic enzyme production by strains ofPellicularia filamentosa to their pathogenicity. Phytopathology 52, 1119–1125.

    CAS  Google Scholar 

  3. Bateman D F 1963 The “macerating enzyme” ofRhizoctonia solani. Phytopathology 53, 1178–1186.

    CAS  Google Scholar 

  4. Bateman D F 1966 Hydrolytic and trans-eliminative degradation of pectic substances by extracellular enzymes ofFusarium solani f, phaseoli. Phytopathology 56, 236–244.

    Google Scholar 

  5. Chambers S C and Flentje N T 1967 Studies on oat-attacking and wheat-attacking isolates ofOphiobolus graminis in Australia. Aust. J. Biol. Sci. 20, 927–940.

    Google Scholar 

  6. Deacon J W 1974 Further studies onPhialophora radicicola andGaeumannomyces graminis on roots and stem bases of grasses and cereals. Trans. Br. Mycol. Soc. 63, 307–327.

    Google Scholar 

  7. Garrett S D 1976 Influence of nitrogen on cellulolysis rate and saprophytic survival in soil of some cereal foot-rot fungi. Soil Biol. Biochem. 8, 229–234.

    CAS  Google Scholar 

  8. Holden J 1976 Infection of wheat seminal roots by varieties ofPhalophora radicicola andGaeumannomyces graminis. Soil Biol. Biochem. 8, 109–119.

    Article  Google Scholar 

  9. Holden M and Ashby M 1978 Polygalacturonase production in liquid culture by isolates ofGaeumannomyces graminis and Phialophora-like fungi from cereal roots. Trans. Brit. Mycol. Soc. 71, 499–501.

    CAS  Google Scholar 

  10. Martyniuk S and Myśków W 1983 Das Vorkommen derGaeumannomyces graminis undPhialophora radicicola-Stämme auf Getreidewurzeln von verschiedenen Standorten und die pektinolytische Aktivität dieser Pilze. Zbl. Mikrobiol. 138, 465–473.

    CAS  Google Scholar 

  11. Martyniuk S 1986 Ekologia i właściwości fitopatogena korzeni zbózGaeumannomyces graminis (Sacc) Arx and Olivier i grzybów pokrewnych z rodzaju Phialophora. Ph.D. Thesis. Institute of Soil Science and Plant Cultivation Press, R(208), Puławy, Poland.

    Google Scholar 

  12. Pearson V 1974 Virulence and cellulolytic enzyme activity of isolates ofGaeumannomyces graminis. Trans. Brit. Mycol. Soc. 63, 199–202.

    Google Scholar 

  13. Sherwood R T 1966 Pectin lyase and polygalacturonase production byRhizoctonia solani and other fungi. Phytopathology 56, 279–286.

    CAS  Google Scholar 

  14. Szyszko E 1971 Instrumentalne metody analityczne. PZWL, Warszawa, 480 p.

    Google Scholar 

  15. Weste G 1970 Extra-cellular enzyme production by various isolates ofOphiobolus graminis andO. graminis var.avenae.I. Enzymes produced in culture. Phytopathol. Z. 67, 189–204.

    CAS  Google Scholar 

  16. Weste G 1978 Comparative pathogenicity of six root parasites towards cereals. Phytopathol. Z. 93, 41–55.

    Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Martyniuk, S. Pectolytic enzymes activity and pathogenicity ofGaeumannomyces graminis var.tritici and related Phialophora-like fungi. Plant Soil 107, 19–23 (1988). https://doi.org/10.1007/BF02371539

Download citation

Key words

  • Gaeumannomyces graminis
  • pectolytic enzymes
  • Phialophora graminicola
  • Phialophora sp.
  • virulence
  • wheat