Effects of the herbicides Lindex and Simazine on chloroplast and nodule development, nodule activity, and grain yield inLupinus albus L.
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Field and laboratory investigations were carried out to determine the influence of two commercial herbicides Lindex and Simazine on symbiotic N2 fixation, the photosynthetic apparatus, percentage of proteins, and grain yield of lupins (Lupinus albus L. cv. Multolupa). The herbicides were added (3 kg commercial product in 9001 per ha) two weeks after sowing. The nodulated roots were tested for nitrogenase activity by the acetylene reduction assay (ARA) at the first and second flowering. The ARA values decreased with herbicide application. The decrease was smaller in the Simazine treatments with inoculated seeds. The ARA for plants treated with Lindex did not increase with inoculation. Nitrogenase activity was greater in the non-inoculated plants growing together with weeds. The effect of Simazine on the photosynthetic apparatus proved to be more toxic than that of Lindex, not only considerably reducing the size of the chloroplasts but also affecting the grana structure. The latter appeared as an electron-dense compact mass. There were no visible alterations in the photosynthetic apparatus of plants treated with Lindex. However starch, which was not present in the control leaves, accumulated.
No ultrastructural changes were observed in the nodule cells treated with Lindex. Simazine altered nodule cells by causing vesicle formation, degeneration of bacteria, and by decreasing the number of N2-fixing bacteroids.
The grain yield decreased with both herbicide treatments, the decrease being lower in the inoculated plants, especially in the Simazine treatment.
Our results suggest that each herbicide had a different effect on N2 fixation, the photosynthetic apparatus and grain yield. Laboratory studies indicated that cyanazine and simazine did not adversely affect the growth of Bradyrhizobium.
Key wordsacetylene reduction Bradyrhizobium chloroplasts herbicides lupins
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- Avrov O E 1966 Effect of herbicides on nodule bacteria and nodule formation in legumes. Dokl. Uses. Akad. Sel. Khuz. Nauk. 3, 16–19. Abstract in Soils Fert 29, 454.Google Scholar
- Bethlenfalvay G J, Norris R F and Phillips D A 1979 Effect of bentazon, a Hill reaction inhibitor, on symbiotic nitrogenfixing capability and apparent photosynthesis. Plant Physiol. 63, 213–215.Google Scholar
- Bethlenfalvay G J and Phillips D A 1977 Effect of light intensity on efficiency of carbon dioxide and nitrogen reduction inPisum sativum L. Plant Physiol. 60, 868–871.Google Scholar
- Bethlenfalvay G J and Phillips D A 1978 Interactions between symbiotic nitrogen fixation, combined-N application and photosynthesis inPisum sativum. Physiol. Plant. 42, 119–123.Google Scholar
- Bollish K P, Dunigan E P and Jadi A W M 1985 Effects of seven herbicides on N2(C2H2) fixation by soybeans. Weed Sci. 33, 427–430.Google Scholar
- Comisión de Métodos Analíticos del Instituto Nacional de Edafología y Agrobiología “José Ma Albareda” 1973 pH, Materia Orgánica y Nitrógeno. Anal. de Edaf. y Agrobiol. 32, 1153–1172.Google Scholar
- Comisión de Métodos Analíticos del Instituto National de Edafología y Agrobiología “José Ma Albareda” 1976 Potasio, Calcio y Magnesio. Anal. de Edaf. y Agrobiol. 35, 813–8824.Google Scholar
- Comisión de Métodos Analíticos de Instituto Nacional de Edafología “José Ma Albareda” 1982 Fósforo asimilable. Anal. de Edaf. y Agrobiol. 40, 1331–1344.Google Scholar
- Dunigan E P, Frey J P, Allen L D Jr and McMahon A 1972 Herbicidal effects on the nodulation ofGlycine max (1) Merrill. Agron. J. 64, 806–808.Google Scholar
- Fedke C 1982 Biochemistry and Physiology of HerbicideAction. 1st ed. Springer Verlag, Berlin pp 25, 35.Google Scholar
- De Felipe M R, Pozuelo J M, Lucas M M and Fernández del Campo F 1985 The effect of isoproturan on root growth and ultrastructure of the photosynthetic apparatus on two wheat cultivars and a weed. Physiol. Plant. 66, 563–568.Google Scholar
- Ghinea L 1982 Analele Institutului de Cercetari pentru cereale si Plaste Technique. Fundulea 49, 333–346.Google Scholar
- Golvano M P, De Felipe M R and Cintas M A 1982 Influence of nitrogen sources on chloroplast development in wheat seedlings. Physiol. Plant. 56, 353–360.Google Scholar
- Hardy R F W, Havelka V D 1975 Photosynthate as a major factor limiting nitrogen fixation by field-grown legumes with emphasis on soybeans.In Symbiotic Nitrogen Fixation in Plants. ed. P S Nutman. pp 421–439. Intern. Biol. Programe. Vol. 7. Cambridge Univ. Press.Google Scholar
- Kecskés M, Borbély J 1977 Xenobiotics and soil microbiota affected by xenobiotic interactions. VI Lupin-Rhizobium symbiosis and herbicide combinations. Acta Bot. Acad. Sci. Hungria 23 (3–4), 357–360.Google Scholar
- Kumar S, Pahwa S K, Promila K and Sharma H R 1981 Effect of Simazine and prometryne on the growth and nodulation of chickpea (Cicer arietinum L.) J. Agric. Sci., Camb. 97, 663–668.Google Scholar
- Lakenen E and Enviö R 1971 A comparison of eight extractants for the determination of plant available micronutrients in soils. Acta Agr. Fenn. 123, 223–232.Google Scholar
- Parker F S 1971. Applications of Infrared Spectroscopy in Biochemistry, Biology, and Medicine. Adam Hilger. London 1971. pp 43–45.Google Scholar
- Rennie R J and Dubetz S 1984 Effect of fungicides and herbicides on nodulation and N2 fixation in soybean fields lacking indigenousRhizobium japonicum. Agron. J. 76, 451–454.Google Scholar
- Ruiz Argüeso T, Cabrera E and Barate M 1981 Selection of simbiotically energy-efficient strains ofRhizobium japonicum by their ability to induce a H2-uptake hydrogenase in the free-living state. Arch. Microbiol. 128, 275–279.Google Scholar
- Tweedy A J and Ries K S 1967 Effect of Simazine on nitrate reductase activity in corn. Plant Physiol. 42, 280–282.Google Scholar
- Van Rensen J J S 1982 Molecular mechanisms of herbicide action near photosynthesis II. Physiol. Plant. 54, 515–521.Google Scholar
- Wu M T, Singh B, Salunkhe D K 1971 Influence of s-triazines on some enzymes of carbohydrates and nitrogen metabolism in leaves of Pea (Pisum sativum L.) and sweet corn (Zea mays L.). Plant Physiol. 48, 517–520.Google Scholar
- Wu M T, Singh B and Salunkhe D K 1972 Effect of s-triazines on protein synthesis in leaves of peas (Pisum sativum L.) and sweet corn (Zea mays L.) and on the ultrastructure of pea cotyledons. Experientia 28, 1002–1003.Google Scholar