Plant and Soil

, Volume 99, Issue 2–3, pp 241–253 | Cite as

Differential effects of low concentrations of aluminium on the growth of four genotypes of white clover

  • S. C. Jarvis
  • D. J. Hatch


The effects of aluminium (Al3+) on the growth of four cultivars of white clover dependent upon NO3−N were examined. Plants were grown in flowing solution culture with carefully maintained low concentrations (0, 12.5, 25 and 50 mmolm−3) of Al, and with P and pH (4.5) also held constant and appropriately low. A three-week treatment period resulted in major effects on the growth and elemental composition of shoots and roots at all concentrations of added Al. There were inherent differences between the cultivars in growth but the relative effects of Al were similar in each case. Examination by S.E.M. and x-ray microanalysis of one cultivar grown at 50 mmolm−3 Al, indicated that Al in the roots was associated with P, especially in old, outer epidermal cells. Aluminium reduced NO3 uptake and there were significant effects of Al on nitrate reductase activity (NRA). In contrast to the other characteristics, there were differential effects between the cultivars in NRA, both in the presence and absence of Al.

Key words

Aluminum Flowing solution culture Genotype Nitrate reductase Trifolium repens L. White clover 


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  1. 1.
    Andrew C S, Johnson A D and Sandland R L 1973 Effect of aluminium on the growth and chemical composition of some tropical and temperate pasture legumes. Aust. J. Agric. Res. 24, 325–339.Google Scholar
  2. 2.
    Bar Akiva A and Saigiv J 1967 Nitrate reductase in the citrus plant. Physiol. Plant. 20, 500–507.Google Scholar
  3. 3.
    Blamey F P C, Edwards D G and Asher C J 1983 Effects of aluminium, OH:Al and P:Al molar ratios, and ionic strength on soybean root elongation in solution culture. Soil Sci. 136, 197–207.Google Scholar
  4. 4.
    Bollard E G 1983 Involvement of unusual elements in plant growth and nutrition.In Inorganic Plant Nutrition. Eds. A Lauchli and R L Bieleski. Springer-Verlag, Berlin, 1983. pp 695–744.Google Scholar
  5. 5.
    Burdon J J 1980 Intra-specific diversity in a natural population ofTrifolium repens L., J. Ecol. 68, 717–735.Google Scholar
  6. 6.
    Carvalho De M M, Edwards D G, Andrew C S and Asher C J 1981 Aluminium toxicity, nodulation, and growth ofStylosanthes species. Agron. J. 73, 261–265.Google Scholar
  7. 7.
    Clarkson D T 1966 Effect of aluminium on the uptake and metabolism of phosphorus by barley seedlings. Plant Physiol. 41, 165–172.Google Scholar
  8. 8.
    Clarkson D T 1967 Interactions between aluminium and phosphorus on root surfaces and cell wall material. Plant and Soil 27, 347–356.CrossRefGoogle Scholar
  9. 9.
    Clement C R, Hopper M J, Canaway R K and Jones L H P 1974 A system for measuring the uptake of ions by plants from flowing solutions of controlled composition. J. Expt. Bot. 25, 81–99.Google Scholar
  10. 10.
    Foy C D, Chaney R L and White M C 1978 The physiology of metal toxicity in plants. Annu. Rev. Plant Physiol. 29, 511–566.CrossRefGoogle Scholar
  11. 11.
    Helyar K R and Anderson A J 1970 Responses of five pasture species to phosphorus, lime, and nitrogen on an infertile acid soil with a high phosphate sorption capacity. Aust. J. Agric. Res. 21, 677–692.CrossRefGoogle Scholar
  12. 12.
    Helyar K R and Anderson A J 1971 Effects of lime on the growth of five species, on aluminium toxicity, and on phosphorus availability. Aust. J. Agric. Res. 22, 701–721.CrossRefGoogle Scholar
  13. 13.
    Huett D O and Menary R C 1980 Aluminium distribution in freeze-dried roots of cabbage, lettuce and Kikuyu grass by energy-dispersive x-ray analysis. Aust. J. Plant Physiol. 1980 7, 101–111.Google Scholar
  14. 14.
    Jarvis S C and Hatch D J 1985a Rates of hydrogen ion efflux by nodulated legumes grown in flowing solution culture with continuous pH monitoring and adjustment. Ann. Bot. 55, 41–51.Google Scholar
  15. 15.
    Jarvis S C and Hatch D J 1985b The-effects of aluminium on the growth of white clover dependent upon fixation of atmospheric nitrogen. J. Expt. Bot. 36, 1075–1086.Google Scholar
  16. 16.
    Jarvis S C and Hatch D J 1986 The effects of low concentrations of aluminium on the growth and uptake of nitrate-N by white clover. Plant and Soil 95, 43–55.CrossRefGoogle Scholar
  17. 17.
    Lewis O A M, Watson E F and Hewitt E J 1982 Determination of nitrate reductase activity in barley leaves and roots. Ann. Bot. 49, 31–37.Google Scholar
  18. 18.
    Munns D N 1965 Soil acidity and growth of a legume II. Reactions of aluminium and phosphate in solution and effects of aluminium, phosphate, calcium and pH onMedicago sativa L. andTrifolium subterraneum L. in solution culture. Aust. J. Agric. Res. 757–766.Google Scholar
  19. 19.
    Murphy H E, Edwards D G and Asher C J 1984 Effects of aluminium on nodulation and early growth of four tropical pasture legumes. Aust. J. Agric. Res. 35, 663–673.CrossRefGoogle Scholar
  20. 20.
    Osborne G J, Prateley J E and Stewart W P 1981 The tolerance of subterranean clover (Trifolium subterraneum L.) to aluminium and manganese. Field Crops Res. 3, 347–358.Google Scholar
  21. 21.
    Rasmussen H P 1968 Entry and distribution of aluminium inZea mays: electron microprobe x-ray analysis. Planta 81, 28–37.CrossRefGoogle Scholar
  22. 22.
    Snaydon R W 1962a Micro-distribution ofTrifolium repens L. and its relation to soil factors. J. Ecol. 50, 133–143.Google Scholar
  23. 23.
    Snaydon R W 1962b The growth and competitive ability of contrasting natural populations ofTrifolium repens L. on calcareous and acid soils. J. Ecol. 50 439–447.Google Scholar
  24. 24.
    Tennant D 1975 A test of a modified line intersect method of estimating root length. J. Ecol. 63, 995–1001.Google Scholar
  25. 25.
    Whitehead D C Yield of white clover and its fixation of nitrogen as influenced by nutritional and soil factors under controlled environment conditions. J. Sci. Fd. Agric. 33, 1227–1234.Google Scholar
  26. 26.
    Wood M, Cooper J E and Holding A J 1984a Soil acidity factors and nodulation ofTrifolium repens. Plant and Soil 78, 367–379.Google Scholar
  27. 27.
    Wood M, Cooper J E and Hoolding A J 1984b Aluminium toxicity and nodulation ofTrifolium repens. Plant and Soil 78, 381–391.Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • S. C. Jarvis
    • 1
  • D. J. Hatch
    • 1
  1. 1.The Animal and Grassland Research InstituteHurley, MaidenheadUK

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