Plant and Soil

, Volume 103, Issue 1, pp 45–50 | Cite as

Effect of combined nitrogen on nitrogen fixation of soybean (Glycine max L. Merill.) as affected by cultivar and rhizobial strain

  • R. Senaratne
  • C. Amornpimol
  • G. Hardarson


Nine strains ofRhizobium japonicum (Bradyrhizobium japonicum) were screened for nitrogen fixation in combination with two cultivars of soybean, Chippewa and Dunadja at two levels of nitrogen fertilization,i.e. 20 kg N ha−1 and 100 kg N ha−1 using15N isotope dilution method. Plant dry weight, nitrogen yield, percent N derived from atmosphere (% Ndfa) and total amount of N2 fixed were strongly influenced by the specific combination of host-genotype and rhizobial strain. The interaction between the host and microsymbiont were so specific that accurate prediction of symbiotic performance of either partner in relation to a particular host or strain of Rhizobium appears impossible. The sensitivity of nitrogen fixation to combined nitrogen was furthermore dependent upon both the rhizobial strain and host-Rhizobium association. It is therefore, inadvisable to describe a certain bacterium as being fully effective/ineffective or sensitive/tolerant to combined nitrogen, except in relation to a particular host-genotype. There is scope for obtaining promising host-Rhizobium associations tolerant to combined nitrogen through exploiting the interactive effects between the host-genotypes and strains of Rhizobium.

Key words

Glycine max isotope dilution method 15nitrogen nitrogen fixation Rhizobium soybean 


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Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • R. Senaratne
    • 1
  • C. Amornpimol
    • 1
  • G. Hardarson
    • 1
  1. 1.Soils Unit, Joint FAO/IAEA ProgrammeIAEA LaboratorySeibersdorfAustria

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