Plant and Soil

, Volume 103, Issue 1, pp 45–50 | Cite as

Effect of combined nitrogen on nitrogen fixation of soybean (Glycine max L. Merill.) as affected by cultivar and rhizobial strain

  • R. Senaratne
  • C. Amornpimol
  • G. Hardarson
Article

Abstract

Nine strains ofRhizobium japonicum (Bradyrhizobium japonicum) were screened for nitrogen fixation in combination with two cultivars of soybean, Chippewa and Dunadja at two levels of nitrogen fertilization,i.e. 20 kg N ha−1 and 100 kg N ha−1 using15N isotope dilution method. Plant dry weight, nitrogen yield, percent N derived from atmosphere (% Ndfa) and total amount of N2 fixed were strongly influenced by the specific combination of host-genotype and rhizobial strain. The interaction between the host and microsymbiont were so specific that accurate prediction of symbiotic performance of either partner in relation to a particular host or strain of Rhizobium appears impossible. The sensitivity of nitrogen fixation to combined nitrogen was furthermore dependent upon both the rhizobial strain and host-Rhizobium association. It is therefore, inadvisable to describe a certain bacterium as being fully effective/ineffective or sensitive/tolerant to combined nitrogen, except in relation to a particular host-genotype. There is scope for obtaining promising host-Rhizobium associations tolerant to combined nitrogen through exploiting the interactive effects between the host-genotypes and strains of Rhizobium.

Key words

Glycine max isotope dilution method 15nitrogen nitrogen fixation Rhizobium soybean 

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References

  1. Afza R, Hardarson G, Zapata F and Danso S K A 1987 Effect of delayed soil and foliar N fertilization on yield and N2 fixation of soybean. Plant and Soil 97, 361–368.Google Scholar
  2. Bremner J M 1965 Total Nitrogen.In Methods of Soil Analysis. Ed. C A Black. pp 1149–1178. American Society of Agronomy. Wisconsin.Google Scholar
  3. Carroll B J, McNeil D L and Gresshoff P M 1985 A supernodulation and nitrate-tolerant symbiotic (nts) soybean mutant. Plant Physiol 78, 34–40.Google Scholar
  4. Copeland R and Pate J S 1969 Nitrogen metabolism of nodulated white clover in presence and absence of nitrate nitrogen.In White Clover Research. Occ. Symp. No. 6. Ed. J Lowe, British Grassland Society, pp 71–77.Google Scholar
  5. Eaglesham A R J, Minchin F R, Summerfield R J, Dart P J, Huxley P A and Day J M 1977 Nitrogen nutrition of cowpea (Vigna unguiculata) III. Distribution of nitrogen within effectively nodulated plants. Exp. Agric. 13, 369–380.Google Scholar
  6. Fiedler R and Proksch G 1975. The determination of nitrogen-15 by emission and mass spectrometry in biochemical analysis: A review. Anal. Chim. Acta 78, 1–62.CrossRefGoogle Scholar
  7. Fried M and Middelboe V 1977 Measurement of the amount of nitrogen fixed by a legume crop. Plant and Soil 47, 713–715.CrossRefGoogle Scholar
  8. Ham G E and Caldwell A C 1978 Fertilizer placement effects on soybean seed yield, N2 fixation and33P uptake. Agron. J. 70, 779–783.Google Scholar
  9. Hardarson G, Zapata F and Danso S K A 1984a Field evaluation of symbiotic nitrogen fixation by rhizobial strains using15N methodology. Plant and Soil 82, 369–375.Google Scholar
  10. Hardarson G, Zapata F and Danso S K A 1984b Effect of plant genotype and nitrogen fertilizer on symbiotic nitrogen fixation by soybean cultivars. Plant and Soil 82, 397–405.Google Scholar
  11. Harper J E and Gibson A H 1984 Nitrate inhibition of nodulation among soybean cultivars x Rhizobium strain combination.In Advances in Nitrogen Fixation. Eds. C Veeger and W E Newton. p. 537. Nijhoff/Junk publishers, Amsterdam.Google Scholar
  12. Koermendy A and Eaglesham A R J 1984 The effect of starter N on soybean nodulation.In Advances in Nitrogen Fixation. Eds. C Veeger and W E Newton. p. 539. Nijhoff/Junk Publishers, Amsterdam.Google Scholar
  13. Manhart J R and Wong P P 1980 Nitrate effect on nitrogen fixation (Acetylene reduction): Activities of legume root nodules induced by Rhizobia with varied nitrate reductase activities. Plant Physiol 69, 502–505.Google Scholar
  14. Maier R J and Brill W J 1978 Mutant strains ofRhizobium japonicum with increased ability to fix nitrogen for soybean. Science 201, 448–450.Google Scholar
  15. Mytton L R 1975 Plant genotype Rhizobium strain interactions in white clover. Ann. Appl. Biol. 80, 103–107.PubMedGoogle Scholar
  16. Mytton L R, El-Sherbeeny M H and Lawes D A 1977 Symbiotic variability inVicia faba. 3. Genetic effects of host plant, Rhizobium strain and of host x strain interaction. Euphytica 26, 785–791.CrossRefGoogle Scholar
  17. Pate J S and Atkins C A 1983 Nitrogen uptake, transport and utilization.In Nitrogen Fixation Vol. 3. Legumes. Ed. W J Broughton. pp 245–298. Clarendon Press, Oxford.Google Scholar
  18. Rennie R J, Rennie D A and Fried M 1978 Concepts of15N usage in dinitrogen fixation studies.In Isotope studies in biological nitrogen fixation. IAEA pp 107–133.Google Scholar
  19. Rennie R J, Dubetz S, Bole J B and Muendel H H 1982 Dinitrogen fixation measured by15N isotope dilution in two Canadian soybean cultivars. Agron. J. 74, 725–730.Google Scholar
  20. Richards J E and Soper R J 1979 Effect of N fertilizer on yield, protein content, and symbiotic nitrogen fixation in faba beans. Agron. J. 71, 807–811.Google Scholar
  21. Ruschel A P, Vose P B, Victoria R L and Salati 1979 Comparison of isotope techniques and non-nodulating isolines to study the effect of ammonium fertilization on dinitrogen fixation in soybean (Glycine max). Plant and Soil 54, 513–525.Google Scholar
  22. Sims A P, Folkes B F, Barber D J and Walls D 1983 The relative importance of nitrogen assimilation and dinitrogen fixation in the economy of the pea crop.In Temperature Legumes—Physiology, Genetics and Nodulation. Eds. D G Jones and D R Davies. 159–174. Pitman. Books Ltd., London.Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • R. Senaratne
    • 1
  • C. Amornpimol
    • 1
  • G. Hardarson
    • 1
  1. 1.Soils Unit, Joint FAO/IAEA ProgrammeIAEA LaboratorySeibersdorfAustria

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