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Nuclear antigens in the HeLa cell cycle

Molecular and Cellular Biochemistry volume 67, pages 101–110 (1985)Cite this article

Summary

Antisera to 0.35 M NaCl extracts and residues of S phase HeLa nuclei were reacted with electrophoretically separated proteins from the nuclei or nuclear material of HeLa cells synchronized in G1, S, G2 or M phases of the cell cycle. Quantitative evaluation of the peroxidase-antiperoxidase stained nitrocellulose transfers (Western blots) revealed significant changes in the quantities of nuclear non-histone proteins during the cell cycle. Immunochemical staining of electrophoretically separated nuclear antigens permits their selective detection in minute quantities and in the presence of many additional proteins.

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Author notes

  1. Zainy M. Banjara

    Present address: School of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia

Authors and Affiliations

  1. Departments of Biochemistry, Pathology and the A. B. Hancock, Jr. Memorial Laboratory of the Vanderbilt University Cancer Center, Vanderbilt University School of Medicine, 37232, Nashville, TN, USA

    Zainy M. Banjara, Robert C. Briggs & Lubomir S. Hnilica

  2. Department of Biochemistry and Molecular Biology, University of Florida School of Medicine, 32610, Gainesville, FL, USA

    Janet L. Stein & Gary S. Stein

Authors
  1. Zainy M. Banjara
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  2. Robert C. Briggs
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  3. Lubomir S. Hnilica
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  4. Janet L. Stein
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  5. Gary S. Stein
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Banjara, Z.M., Briggs, R.C., Hnilica, L.S. et al. Nuclear antigens in the HeLa cell cycle. Mol Cell Biochem 67, 101–110 (1985). https://doi.org/10.1007/BF02370168

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  • DOI: https://doi.org/10.1007/BF02370168

Keywords

  • antigens
  • cell cycle
  • chromosomal proteins
  • HeLa