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Somatic Cell and Molecular Genetics

, Volume 22, Issue 2, pp 151–157 | Cite as

Immortalization of four new fanconi anemia fibroblast cell lines by an improved procedure

  • P. M. Jakobs
  • P. Sahaayaruban
  • H. Saito
  • C. Reifsteck
  • S. Olson
  • H. Joenje
  • R. E. Moses
  • M. Grompe
Brief Communication

Abstract

Fanconi anemia (FA) is an autosomal recessive disease characterized by birth defects, progressive bone marrow failure and increased risk for leukemia. FA cells display chromosome breakage and increased cell killing in response to DNA crosslinking agents. At least 5 genes have been defined by cell complementation studies, but only one of these, FAC has been cloned to date. Efforts to map and isolate new FA genes by functional complementation have been hapered by the lack of immortalized FA fibroblast cell lines. Here we report the use of a novel immortalization strategy to create 4 new immortalized FA fibroblast lines, including one from the rare complementation group D.

Keywords

Fanconi Anemia Fibroblast Cell Line Bone Marrow Failure Complementation Group Chromosome Breakage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • P. M. Jakobs
    • 1
  • P. Sahaayaruban
    • 1
  • H. Saito
    • 2
  • C. Reifsteck
    • 1
  • S. Olson
    • 1
  • H. Joenje
    • 3
  • R. E. Moses
    • 1
  • M. Grompe
    • 1
    • 4
  1. 1.Department of Molecular and Medical GeneticsOregon Health Sciences UniversityPortland
  2. 2.Department of Human Biological Chemistry and GeneticsThe Univeristy of Texas Medical Branch atGalveston
  3. 3.Institute of Human GeneticsFree UniversityAmsterdamThe Netherlands
  4. 4.Department of Molecular and PediatricsOregon Health Sciences UniversityPortland

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