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Somatic Cell and Molecular Genetics

, Volume 22, Issue 2, pp 135–144 | Cite as

Cyclin D1 induced apoptosis maintains the integrity of the G1/S checkpoint following ionizing radiation irradiation

  • Francisco S. Pardo
  • Mei Su
  • Carmia Borek
Article

Abstract

Cell cycle “checkpoints” help to ensure the integrity of normal cellular functions prior to replicative DNA synthesis and/or cell division. Cell kinetic abnormalities, particularly arrests at the G1/S and G2/M cell cycle checkpoints, are induced following exposure to ionizing radiationin vitro. Following irradiation, cellular signaling pathways may lead to G1 arrest and/or apoptosis at the G1/S cell cycle transition point. Transfection of cyclin D1, a G1/S cyclin, into a rat embryo cells (REC) results in cellular populations that overexpress cyclin D1, are transformed morphologically, demonstrate an increased incidence of apoptosis, and are tumorigenic in immune-deficient mice. Despite such phenotypic changes, transfected cell populations maintain the itegrity of the G1 checkpoint following ionizing radiation. The transfected cells overexpressing Cyclin D1 have a statistically significant increase in the incidence of apoptosis as compared to parental REC strains or mock-transfected REC. The work provides further evidence of Cyclin D1 playing a critical role in maintaining the integrity of the G1/S checkpoint, via the activation of apoptotic pathways following exposure to ionizing radiationin vitro.

Keywords

Cell Cycle Transition Point Apoptotic Pathway Phenotypic Change Cycle Checkpoint 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

REC

ratembryo cell

Cy D1

pop, cyclin D1-transfected cell population

pMMTVPRAD-1

cyclin D1 expression vector

pCMV

neo, neomycin expression vectoi

cGy

centigray (1 rad)

Gy

Gray (100 rad)

MID, SCID

Severe Combined Immunodeficiency mice; nu/nu. nude mice

NIH III

triple deficient mice

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Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Francisco S. Pardo
    • 1
  • Mei Su
    • 1
  • Carmia Borek
    • 2
  1. 1.Laboratory of Molecular/Tumor Radiation Biology Mass Gen. Hosp/Harvard Medical SchoolTufts University Boston
  2. 2.Department Community HealthTufts University Boston

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