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Somatic Cell and Molecular Genetics

, Volume 22, Issue 6, pp 499–509 | Cite as

Generation of a panel of radiation-reduced hybrids containing human 11q22-23 fragments bearing aHPRT selective marker: Identification of hybrids carrying various subregions around the ataxia-telangiectasia locus

  • Yosuke Ejima
  • Mitsuo Oshimura
  • Masao S. Sasaki
Brief Communication

Abstract

A human-mouse monochromosomal hybrid that contains a human t(X;11) translocated chromosome carrying pter→q23 segment of chromosome 11 was used to construct a panel of radiation-reduced hybrids. The hypoxhanthine phosphoribosyltransferase (HPRT) gene located close to the translocation breakpoint was used as a marker to select for the hybrids that preferentially retain the 11q22-23 region. Twenty-three HAT-resistant hybrids were isolated and screened by polymerase chain reaction (PCR) for the retention of 31 loci on 11q22-23 region. Among the 14 hybrids that had breakpoints within the 11q22-23 region, 6 hybrids contained fragments that extend either from centromere or telomere to the 5-Mb region spanned by GRIA4 and FDX, carrying various breakpoints within the region. This subpanel could be a potential resource to analyze the ataxia-telangiectasia disease locus and its neighboring region.

Keywords

Polymerase Chain Reaction Disease Locus Selective Marker Neighboring Region Potential Resource 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Yosuke Ejima
    • 1
  • Mitsuo Oshimura
    • 2
  • Masao S. Sasaki
    • 1
  1. 1.Radiation Biology CenterKyoto University, Yoshida-KonoechoKyoto
  2. 2.Department of Molecular and Cell Genetics, School of Life SciencesTottori UniversityYonagoJapan

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