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A new flow cell for platelet adhesion studies

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Abstract

A flow cell has been designed and tested for the purpose of exposing platelets to various substrates. The design adopts the principle of flow relaminarization by acceleration to dissipate secondary fluid motions and turbulence so that platelet diffusion will be controlled by Brownian diffusion coefficients. To assess the effectiveness of this flow mechanism two critical tests were undertaken. First, hot film anemometry signals were obtained to observe visually the local fluid velocity in the flow cell. Second, in vitro platelet adhesion results were obtained by exposing glass and silane coated glass to platelet suspensions. Surface platelet concentrations for varying exposure times were compared to a theoretical model which is based on a laminar diffusion model. Both tests confirm that hydrodynamically the flow cell behaves in a manner which is consistent with that of a fully developed, laminar flow with a constant diffusion coefficient.

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Authors and Affiliations

  1. Department of Mechanical Engineering, University of Waterloo, Waterloo, Ontario, Canada

    Allan B. Strong, Caroline Ledain & Brain E. Thompson

  2. Department of Surgical Research, The Hospital for Sick Children, 555 University Avenue, M5G 1X8, Toronto, Ontario, Canada

    Allan B. Strong, Darryl R. Absolom & Walter Zingg

  3. Department of Mechanical Engineering, University of Toronto, Toronto, Ontario, Canada

    Darryl R. Absolom & Oakt Hum

  4. Institute of Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada

    Walter Zingg

Authors
  1. Allan B. Strong
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  2. Darryl R. Absolom
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  3. Walter Zingg
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  4. Oakt Hum
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  5. Caroline Ledain
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  6. Brain E. Thompson
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Strong, A.B., Absolom, D.R., Zingg, W. et al. A new flow cell for platelet adhesion studies. Annals of Biomedical Engineering 10, 71–82 (1982). https://doi.org/10.1007/BF02366999

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