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Ethanol inhibits asialoglycoprotein receptor synthesis but augments its mRNA expression in a human hepatoma cell line

  • Liver, Pancreas, and Biliary Tract
  • Published:
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Abstract

The effect of ethanol on the expression of asialoglycoprotein receptor protein and its mRNA was studied in a human hepatoma cell line, HepG2. The number of asialoglycoprotein receptors on the cell surface was decreased to 60% of the control level, without a loss in affinity, by incubating the cells with 100 mM ethanol. The decrease in cell surface asialoglycoprotein receptors was paralleled by a decrease in total receptor numbers, including intracellular and surface receptors. The internalization of asialoglycoprotein was also diminished, to 44% of that in control cells. The decreases in cell surface receptors and total receptor numbers were partially restored by 2 mM 4-methylpyrazole, suggesting that ethanol metabolites participated in the diminution of asialoglycoprotein receptor expression. However, the steady-state expression of asialoglycoprotein receptor mRNA was increased in ethanol-treated cells and further augmented by a longer ethanol exposure. These paradoxical results, i.e., the decrease of asialoglycoprotein receptor protein and the increase of its mRNA expression, suggest that the reduction in the asialoglycoprotein receptor protein is a post-transcriptional event and that a possible feedback regulatory mechanism may control asialoglycoprotein receptor gene transcription and/or impair the degradation of its mRNA.

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Authors and Affiliations

  1. Department of Internal Medicine, Section 4, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, 060, Sapporo, Japan

    Yutaka Kohgo, Yoshihiro Mogi, Junji Kato, Reiji Nakaya, Masahiro Nakajima, Shinichi Katsuki & Yoshiro Niitsu

Authors
  1. Yutaka Kohgo
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  2. Yoshihiro Mogi
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  3. Junji Kato
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  4. Reiji Nakaya
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  5. Masahiro Nakajima
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  6. Shinichi Katsuki
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  7. Yoshiro Niitsu
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Kohgo, Y., Mogi, Y., Kato, J. et al. Ethanol inhibits asialoglycoprotein receptor synthesis but augments its mRNA expression in a human hepatoma cell line. J Gastroenterol 29, 598–604 (1994). https://doi.org/10.1007/BF02365442

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  • DOI: https://doi.org/10.1007/BF02365442

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