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Biosynthesis and secretion of mucin-related products in Hs746T gastric cancer cells

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Abstract

We have previously studied the biosynthesis and secretion of mucin in the normal human stomach and reported that the tetramer of the 60-kDa subunit of mucin core protein was synthesized and highly glycosylated, and that only high molecular weight mucin was secreted. In this study, we investigated the mucin-related products of a gastric cancer cell line (Hs746T). Analysis of intracellular and extracellular products immunoprecipitated with an anti-apomucin monoclonal antibody revealed that a 110-kDa protein, the dimer of the 55-kDa subunit, was synthesized and secreted. Tunicamycin treatment inhibited the secretion of the 110-kDa protein. These findings suggest thatN-glycosylation may be involved in the secretory mechanism of the mucin-related product.

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  1. First Department of Internal Medicine, Kochi Medical School, Okoh, Nankoku, 783, Kochi, Japan

    Shuichi Sano, Kazuichi Okazaki & Yasutake Yamamoto

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  1. Shuichi Sano
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  2. Kazuichi Okazaki
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  3. Yasutake Yamamoto
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Sano, S., Okazaki, K. & Yamamoto, Y. Biosynthesis and secretion of mucin-related products in Hs746T gastric cancer cells. J Gastroenterol 29, 569–576 (1994). https://doi.org/10.1007/BF02365437

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  • DOI: https://doi.org/10.1007/BF02365437

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