Abstract
The effects of the muscarinic receptor agonist, carbamylcholine chloride (carbachol), on gastrin release and gastrin mRNA levels in human antral mucosa (n=15) were determined. During a-2-h incubation period, carbachol (10−6−10−4M) decreased gastrin mRNA levels to 71±8% (10−6M), 40±8% (10−5M), and 33±5% (10−4M) of control levels. Carbachol (10−5M) decreased intracellular gastrin (from 1634±103 to 1272±126 pg/mg tissue protein), while it increased gastrin release into the medium (from 609±48 to 918±68 pg/ml per mg tissue protein). After 6-and 9-h culture, carbachol gradually increased gastrin mRNA levels, by 96±12% and 126±23%, respectively. Atropine sulfate (10−5 M) completely inhibited the carbachol-induced changes. Cycloheximide markedly decreased tissue gastrin concentration, but increased gastrin mRNA levels, whereas it had no effects on gastrin release. These findings suggested that carbachol may have a time-related biphasic action on human antral gastrin biosynthesis.
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Okazaki, K., Kino, J., Suenaga, K. et al. Effects of carbamylcholine chloride on human antral gastrin mRNA levels. J Gastroenterol 29, 553–558 (1994). https://doi.org/10.1007/BF02365435
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DOI: https://doi.org/10.1007/BF02365435