Advertisement

Annals of Biomedical Engineering

, Volume 8, Issue 3, pp 253–269 | Cite as

Alternating current electrophoresis of human red blood cells

  • Peter C. Y. Chen
Article

Abstract

An a.c. electrophoretic technique in the frequency range between 2–10 Hz, using a rectangular chamber design, has been developed for measuring the surface electric charge density of human red blood cells. With the a.c. approach, mobility can be determined rapidly, electrode polarization is minimized and the construction of the chamber simplified. For frequencies under 10 Hz and a chamber thickness of 150 μm, a theoretical analysis of the electroosmotic flow profile shows that it is almost identical to the d.c. case. With the a.c. method and using a 0.145 N NaCl solution buffered with NaHCO3, the mobility of red cells measured at the lower stationary level is found to be −1.07±0.02 (S.D.) μmsec−1 V−1 cm. The zeta potential and charge density calculated from the mobility are −14.03±0.26 (S.D.) mV and −3603±69 (S.D.) esu cm−2, respectively.

Keywords

Electrophoresis Charge Density Zeta Potential NaHCO3 Electric Charge 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bluh, O. Studies of colloid particles in an alternating-current field.Ann. Phys. 76:177–194, 1925.Google Scholar
  2. 2.
    Bluh, O. Some phenomena occurring with the investigation of colloids in an alternating field.Kolloid Z. 37:267–270, 1925.Google Scholar
  3. 3.
    Brinton, C. C., Jr., and M. A. Lauffer,Electrophoresis, edited by M. Bier. New York: Academic Press, 1959, pp. 428–492.Google Scholar
  4. 4.
    Chapman, D. L. A contribution to the theory of electrocapillarity,Philos. Mag. 25:475–481, 1913.Google Scholar
  5. 5.
    Chen, P. C. Y. A.C. electrophoresis of human red blood cells. Ph.D. thesis, University of California, San Diego, 1978.Google Scholar
  6. 6.
    Debye, P. and E. Huckel.Remarks on a Theorem on the Cataphoretic Migration Velocity of Suspended Particles. Collected papers of Peter T. W. Debye. New York: Interscience Publisher, 1954, pp. 345–354.Google Scholar
  7. 7.
    Delatour, E. and M. Hanss. Micro-electrophoresis in champ alternatif.J. Fr. Biophys. Med. Nucl. 4:173–177, 1977.Google Scholar
  8. 8.
    Gouy, G. Contribution of electric charge at surface of an electrolyte.J. Phys. Radium 9:457, 1910.Google Scholar
  9. 9.
    Grahame, D. C. Effects of dielectric saturation upon the diffuse double layer and the free energy of hydration of ions.J. Chem. Phys. 18:903–909, 1950.CrossRefGoogle Scholar
  10. 10.
    Helmholtz, H. Studies wher elektrische zrenzchicten.Ann. Phys. (Leipzig) 7:337–382, 1879.Google Scholar
  11. 11.
    Henry, D. C. The cataphoresis of suspended particles.Proc. Roy. Soc. London, Ser. A 133: 106–129, 1931.Google Scholar
  12. 12.
    Levich, V. G.Physiochemical Hydrodynamics. New Jersev: Prentice-Hall, 1962.Google Scholar
  13. 13.
    Perrin, J. The phenomenon of Bose-Guilloume and the electrization by contact.Acad. Sci., Paris. Comptes rendus 147:55–56, 1907.Google Scholar
  14. 14.
    Schwann, H. P., G. Schwarz, J. Maczuk, and H. Pauly. On the low frequency dielectric dispersion of colloid particles.J. Phys. Chem. 66:2626–2642, 1962.Google Scholar
  15. 15.
    Seaman, G. V. F. Electrokinetic behavior of red cells.The Red Blood Cell, edited by D. M. Surgenor. New York: Academic Press, 1974, pp. 1136–1222.Google Scholar
  16. 16.
    Sher, L. Mechanical effects of a.c. fields on particles dispersed in a liquid. Ph.D. thesis, University of Pennsylvania, 1963.Google Scholar
  17. 17.
    Stern, O. The theory of the electric double layer.Z. Elektrochem. 30:508–516, 1924.Google Scholar
  18. 18.
    Tenforde, T. Microelectrophoretic studies on the surface chemistry of erythrocytes.Adv. Biol. Med. Phys. 13:43–105, 1970.PubMedGoogle Scholar
  19. 19.
    Vorob’eva, T. A., I. N. Vlodavet, and S. S. Dukhin. Hydrodynamic characteristics of microelectrophoresis and electroosmosis in an alternating electric field.Kolloidn. Zh. 32:No. 2, 189–194, 1970.Google Scholar
  20. 20.
    White, P. The theory of electroosmotic circulation in varying fields.Philos. Mag. 26:49–65, 1958.Google Scholar

Copyright information

© Pergamon Press Ltd. 1981

Authors and Affiliations

  • Peter C. Y. Chen
    • 1
  1. 1.Department of BioengineeringUniversity of California, San DiegoLa Jolla

Personalised recommendations