Annals of Biomedical Engineering

, Volume 11, Issue 6, pp 589–598 | Cite as

Membrane transport in vascular smooth muscle and its relation to normal and altered excitation during hypertension

  • Allan W. Jones
  • Jacquelyn M. Smith
  • David B. Bylund
  • Ellen T. Garwitz
Article

Abstract

Evidence is presented for the operation of a Cl−Cl exchange diffusional transport system in rat aortic smooth muscle. The efflux of36Cl associated with this mechanism is doubled in rats made hypertensive with aldosterone-salt treatment. The residual efflux of Cl is similarly elevated in the hypertensives. This finding supports the hypothesis that increased membrane permeability to Cl is associated with aldosterone hypertension. The agonist-induced increases in aortic effluxes of42K exhibit a 10-fold reduction in the ED50 to norepinephrine in aldosterone hypertensive rats. The possible contributors to this supersensitivity were studied by means of a pharmacologic analysis of the action of competitive (phentolamine) and non-competitive (dibenamine) antagonism of norepinephrine induced increases in42K efflux. The dissociation constant for phentolamine, KB=1×10−8 M, was relatively unaltered in the hypertensive group, as was the dissociation constant for norepinephrine, KA=3.4−5.8×10−7 M. A substantial increase in receptor number and/or transduction efficiency (3–12 fold) was derived from the analysis. It is tentatively concluded that supersensitivity to norepinephrine during aldosterone hypertension may be more closely related to changes in receptor number and/or efficiency than in receptor affinity.

Keywords

Ion transport Supersensitivity Aldosterone hypertension Aorta Competitive antagonist Non-competitive antagonist 

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Copyright information

© Pergamon Press Ltd. 1984

Authors and Affiliations

  • Allan W. Jones
    • 1
  • Jacquelyn M. Smith
    • 1
  • David B. Bylund
    • 1
  • Ellen T. Garwitz
    • 2
  1. 1.Department of PhysiologyUniversity of MissouriColumbia
  2. 2.Department of PharmacologyUniversity of MissouriColumbia

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