Summary
Enzyme-linked immunosorbent assay (ELISA), used in conjunction with a new rapid extraction method, showed that potato leafroll virus (PLRV) concentration in the vascular region of infected potato tubers decreases from the heel to the rose end. Lower virus concentration at the rose than at the heel end was found not only in dormant tubers but also in tubers three weeks after breaking dormancy although the difference was then less pronounced. These results were obtained from plants with both primary and secondary infection by one of two French virus isolates which behave differently in respect of either accumulation in the ants or in their serological properties or both.
References
Arenz, B. & W. Hunnius, 1963. Erfahrungen mit dem Igel-Lange-Test in der Serienarbeit.Bayer. landb. Jb. 40 (1): 122–136.
Bokx, J. A. de, 1975. Virologisch onderzoek gericht op de kwaliteit van pootaardappelen.Bedrijfsontwikkeling 6: 939–941.
Casper, R., 1977. Detection of potato leafroll virus in potato and inPhysalis floridana by enzyme-linked immunosorbent assay (ELISA).Phytopath. Z. 90: 364–368.
Clark, M. F. & A. N. Adams, 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses.J. gen. Virol. 34: 475–483.
Gugerli, P., 1978. The detection of two potato viruses by enzyme-linked immunosorbent assay (ELISA).Phytopath. Z. 92: 51–56.
Gugerli, P., 1979a. Potato virus A and potato leafroll virus: Purification, antiserum production and serological detection in potato and test plants by enzyme-linked immunosorbent assay (ELISA).Phytopath. Z. 96: 97–107.
Gugerli, P., 1979b. Le test immuno-enzymatic ELISA (‘enzyme-linked immunosorbent assay’) et son application pour le diagnostic rapide des viroses de la pomme de terre.Revue suisse Agric. 11 (6) 253–260.
Keller, E. R. & S. Bercès, 1966. Check-testing for virus Y and leafroll in seed potatoes with particular reference to methods of increasing precision with the A6-leaf test for virus Y.Eur. Potato J. 9: 1–14.
Maat, D. Z. & J. A. de Bokx, 1978a. Potato leafroll virus: antiserum preparation and detection in potato leaves and sprouts with enzyme-linked immunosorbent assay (ELISA).Neth. J. Pl. Path. 84: 149–156.
Maat, D. Z. & J. A. de Bokx, 1978b. Enzyme-linked immunosorbent assay (ELISA) for the detection of potato viruses A and Y in potato leaves and sprouts.Neth. J. Pl. Path. 84: 167–173.
Mehrad, M., H. Lapierre & Y. Maury, 1978. Le virus de l’enroulement de la pomme de terre: purification, détection sérologique et dosage dans la plante.C.r. Acad. Sc. Paris 286: 1179–1182.
Münster, J., 1965. Hinweise zu den Igel-Lange- und Solanum A6-Testen im Rahinen der Saatkartoffelanerkennung.Kartoffelbau 16: 215–218.
Voller, A., A. Barlett, D. E. Bidwell, M. F. Clark & A. N. Adams, 1976. The detection of viruses by enzyme-linked immunosorbent assay (ELISA).J. gen. Virol. 33: 165–167.
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Gugerli, P. Potato leafroll virus concentration in the vascular region of potato tubers examined by enzyme-linked immunosorbent assay (ELISA). Potato Res 23, 137–141 (1980). https://doi.org/10.1007/BF02364036
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DOI: https://doi.org/10.1007/BF02364036