Summary
It was found that addition of an equal volume of 0.2% sodium sulphite or a mixture of sodium sulphite and sodium azide (both 0.2%) to expressed leaf sap improved serological detection of viruses and prevented non-specific reactions. Incubations of the preparations at 20–24°C gave better serological detectability than incubating at 10–12°C. If the sap was centrifuged, better results were subsequently obtained if centrifuging had been done at 4200 or 5900 g than at lower values.
This is a preview of subscription content, log in via an institution to check access.
References
Gooding, G. V. & J. P. Tsakiridis, 1971. Sodium azide as a protectant of serological activity of plant viruses.Phytopathology 61: 943–944.
Oertel, C. 1968. Serologischer Virusnachweis als Routinentest und Bestandteil planzenhygienischer Massnahmen in Chryzanthemjang-Pflanzenbetrieb.Z. PflKrankheiten 75: 605–612.
Staszewicz M. & E. Swiniarski, 1975. Influence of some chemical compounds on serological detection of potato viruses X, S, M and Y.Potato Res. 18: 148 (Abstr.).
Staszewicz M. & E. Swiniarski, 1976. Wpływ azydku sodu i siarczynu sodu na serologiczna wykrywalność wirusow X, S, M i Y w liściach i bulwach ziemniaka.Zesz. Probl. Post. Nauk rol. 174: 61–68.
Vulic, M. & B. Arenz, 1962. Über eine verbesserte Arbeitweise zur serologischen Bestimmung des Y-Virus in der Kartoffelpflanze.Nachr. Dt. Pfl Schutzdienst. Braunschweig 14: 65–69.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Staszewicz, M. Treatments that improve serological detection of potato viruses X, S, M and Y. Potato Res 20, 89–93 (1977). https://doi.org/10.1007/BF02362304
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02362304