Skip to main content
SpringerLink
Log in

Colorimetric determination of the total chlorophyll concentrations in potato leaves by liquid scintillation counting

  • Short Communication
  • Published:
Potato Research Aims and scope Submit manuscript

Summary

Extraction of chlorophyll from leaf material with dimethyl sulphoxide allowed measurement of the attenuation of photon detection from a sealed miniature14C standard to be used for total chlorophyll quantification. Comparison between the use of a spectrophotometer and a liquid scintillation counter showed chlorophyll quantification to be accurate by this latter method over a wide range of concentrations. The method does not separate chlorophyllsa andb, but allows the rapid measurement of total chlorophyll concentration, and is suitable for automation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Arnon, D. I., 1949. Copper enzymes in isolated chloroplasts. Polyphenolixdase inBeta vulgaris.Plant Physiology 24: 1–15.

    CAS  Google Scholar 

  • Davies, R. G., 1971.Computer Programming in Quantitative Biology. Academic Press, London.

    Google Scholar 

  • Hiscox, J. D. & G. F. Israelstam, 1979. A method for the extraction of chlorophyll from leaf tissue without maceration.Canadian Journal of Botany 57: 1332–1334.

    CAS  Google Scholar 

  • Hunt, R. & I. T. Parsons, 1974. A computer program for deriving growth functions in plant growth analysis.Journal of Applied Ecology 11: 297–307.

    Google Scholar 

  • Morrison, I. M. & R. E. Brice, 1981. The use of a liquid scintillation counter for the colorimetric determination of carbohydrates.Carbohydrate Research 98: 237–241.

    Article  CAS  Google Scholar 

  • Millard, P. & D. K. L. MacKerron, 1986. The effects of nitrogen application on growth and nitrogen distribution within the potato canopy.Annals of Applied Biology 109: 427–437.

    Google Scholar 

  • Noble, R. C. & J. H. Shand, 1983. The colorimetric estimation of protein by the Lowry technique using a liquid scintillation counter.Journal of Biochemical and Biophysical Methods 8: 223–226.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. D. Robinson

    Present address: Department of Physiology and Crop Production, Scottish Crop Research Institute, DD2 5DA, Invergowrie, Dundee, Scotland

Authors and Affiliations

  1. Department of Soil Fertility, Macaulay Land Use Research Institute, AB9 2QJ, Craigiebuckler, Aberdeen, Scotland

    P. Millard & D. Robinson

Authors
  1. P. Millard
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Robinson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Millard, P., Robinson, D. Colorimetric determination of the total chlorophyll concentrations in potato leaves by liquid scintillation counting. Potato Res 30, 491–494 (1987). https://doi.org/10.1007/BF02361926

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02361926

Additional keywords

Search

Navigation