Skip to main content
Log in

Induction of hydrolytic enzymes and antifungal in vitro activity inS. tuberosum subsp.andigena after infection withFusarium solani f. sp.eumartii

  • Published:
Potato Research Aims and scope Submit manuscript

Summary

Fusarium solani (Mart.) Sacc. f. sp.eumartii (Carp.) Snyder & Hansen is the causative agent of two important types of disease in potato (Solanum tuberosum) plants: wilt in stems and leaves and dry rot in tubers. The aim of this work was to study the molecular response of the potato to fungal attack. Micropropagated plantlets were tested, using a biological assay to detect antifungal proteins. An inhibitory activity of spore germination was detected in protein fractions from infected plantlets 7 days after inoculation with the pathogen. In addition, β-1,3-glucanase activity (glucanase III) was measured in protein fractions with antifungal activity. Induction of hydrolytic enzymes and antifungal activity in infected plantlets ofSolanum tuberosum subp.andigena suggests that it could be a suitable source of resistance genes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Benhamou, N., P.M. Charest & W.R Jarvis, 1989. In: NATO ASI Series, E.C. Tjamos & C. Beckman (Eds), Vascular wilt diseases of plants. Springer-Verlag, Berlin, pp. 95–105.

    Google Scholar 

  • Botta, G.L., M.P. Dimarco, A.L. Melegari, M.A. Huarte & C.A. Barassi, 1994. Potential of aFusarium eumartii culture filtrate on the screening for wilting resistance in potato.Euphytica 80: 63–69.

    Article  Google Scholar 

  • Bowles, D.J., 1990. Defense-related proteins in higher plants.Annual Review of Biochemistry 59: 873–907.

    Article  CAS  PubMed  Google Scholar 

  • Bulcke, M. van den, G. Bauw, C. Castresana, M. van Montagu & J. Vandekerckhove, 1989. Characteriztion of vacuolar and extracellular β-(1,3)-glucanases of tobacco: Evidence for a strictly compartmentalized plant defense system.Proceedings of the National of Academy of Sciences of the United States of America 86: 2673–2677.

    Google Scholar 

  • Cherif, M. & N. Benhamou, 1990. Cytochemical aspects of chitin brakdown during the parasitic action of aTrichoderma sp. onFusarium oxysporum f. sp.radicis-lycopersici.Phytopathology 80: 1406–1414.

    CAS  Google Scholar 

  • Desjardins, A.E., 1992. Genetic approaches to the chemical ecology of phytopathogenicFusarium species. In: D. Bahtnagar, E.B. Lillehoj & D.K. Arora (Eds), Handbook of applied mycology 5: Mycotoxins in ecological systems. Marcel Dekker, New York, Basel, Hong Kong, pp. 333–357.

    Google Scholar 

  • Enyedi, A.J., N. Yalbani, P. Silverman & Y. Raskin, 1992. Signal molecules in systematic plant resistance to pathogens and pests.Cell 70: 879–886.

    Article  CAS  PubMed  Google Scholar 

  • García-Olmedo, F., M.J. Carmona, J.J. López-Fando, J.A. Fernández, A. Castagnaro, A. Molina, C. Hernández Lucas & P. Carbonero, 1992. In: T. Boller & F. Meins (Eds), Plant gene research series: genes involved in plant defense. Springer-Verlag, Berlin, pp. 283–302.

    Google Scholar 

  • Graham, L.S. & M.B. Sticklen, 1994. Plant chitinases.Canadian Journal of Botany 72: 1057–1083.

    CAS  Google Scholar 

  • Kombrink, E., L. Beerhues, K. Hahlbrock & B. Witte, 1991. Differential patterns of expression and cellular localization of chitinase and 1,3-β-glucanases in potato. In: A.H.C. Huang & L. Taiz (Eds), Molecular approaches to compartmentation and metabolic regulation. American Society of Plant Physiology, USA, pp. 243–244.

    Google Scholar 

  • Kombrink, E., M. Scröder & K. Hahlbrock, 1988. Several pathogenesis- related proteins in potato are 1,3-β-glucanases and chitinases.Proceedings of the National of Academy of Sciences of the United States of America 85: 782–786.

    CAS  Google Scholar 

  • Krebs, S.L. & R. Grumet, 1993. Affinity purification and characterization of a β-1,3-glucanase from celery.Plant Science 93: 31–39.

    Article  CAS  Google Scholar 

  • Leach, S.S. & R.E. Webb, 1981. Resistance of selected potato cultivars and clones toFusarium dry rot.Phytopathology 71 (6): 623–629.

    Google Scholar 

  • Mauch, F., B. Mauch-Mani & T. Boller, 1988. Antifungal hydrolases in pea tisse. II. Inhibition of fungal growth by combinations of chitinase and β-1,3-glucanase.Plant Physiology 88: 936–942.

    CAS  Google Scholar 

  • Moreno, M., A. Segura, F. García-Olmedo, 1994. Pseudothionin-St 1, a potato peptide active against potato pathogens.European Journal of Biochemistry 223: 135–139.

    Article  CAS  PubMed  Google Scholar 

  • Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures.Physiologia Plantarum 15: 437–497.

    Google Scholar 

  • Nelson, M., 1944. A photometric adaptation of the Somogy method for the determination of glucose.Journal of Biological Chemistry 153: 375–380.

    CAS  Google Scholar 

  • Punja, Z.K. & Ye-yan Zhang, 1993. Plant chitinases and their roles in resistance to fungal diseases.Journal of Nematology 25: 526–540.

    CAS  Google Scholar 

  • Radtke, W. & A. Escande, 1973. Pathogenicity of isolates of a fungi collection (Fusarium) onSolanum tuberosum cultivars.De la Revista de la Facultad de Agronomía, Argentina XLIX: 61–79.

    Google Scholar 

  • Raikhel, N.V. & H.-I. Lee, 1993. Structure and function of chitin-binding proteins.Annual Review of Plant Physiology and Plant Molecular Biology 44: 591–615.

    Article  CAS  Google Scholar 

  • Reissig, J.L., J.L. Strominger & L.F. Leloir, 1955. A modified colorimetric method for the estimation of N-acetylaminosugars.Journal of Biological Chemistry 217: 959–966.

    CAS  PubMed  Google Scholar 

  • Stintzi, A., T. Heitz, V. Prasad, S. Wiedemann-Merdinoglu, S. Kauffmann, P. Geoffroy, M. Legrand & B. Fritig, 1993. Plant “pathogenesis-related” proteins and their role in defense against pathogens.Biochimie 75: 687–706.

    Article  CAS  PubMed  Google Scholar 

  • Tozzini, A.C., M.F. Ceriani, M.V. Saladrigas & H.E. Hopp, 1991. Extreme resistance to infection by potato virus X in genotypes of wild tuber-bearingSolanum species.Potato Research 34: 317–324.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Godoy, A.V., Olivieri, F.P. & Casalongue, C.A. Induction of hydrolytic enzymes and antifungal in vitro activity inS. tuberosum subsp.andigena after infection withFusarium solani f. sp.eumartii . Potato Res 39, 259–266 (1996). https://doi.org/10.1007/BF02360917

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02360917

Additional keywords

Navigation