Summary
The effect of liquid and solid media on the growth of micropropagated potato shoots (cvs Spunta, Kennebec and Huinkul) was studied in relation to the availability of nutrients and water in the culture medium. Nitrogen and sugar assimilation in the shoot and the water content of the shoots were evaluated. The water content of the plant tissues was not affected by the physical state of the medium. The liquid medium caused a greater accumulation of dry matter in the shoot without changing assimilate partition. The liquid medium increased shoot growth without affecting the number of nodes. The higher growth rate of shoots in liquid medium was correlated with increases in their organic nitrogen and sugar contents, indicating that nutrient assimilation was favoured in liquid media.
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Avila, A. de L., S.M. Pereyra, D.J. Collino & J.A. Argüello, 1994. Effects of nitrogen source on growth and morphogenesis of three micropropagated potato cultivars.Potato Research 37: 161–168.
Bouniols, A., 1974. Neoformation de bourgeons in vitro à partir de fragments de racime d'endive (Cichorium intybus L.): influence du degré d'hydratation des tissus, et ses conséquences sur la composition en acides aminés.Plant Science Letters 2: 363–371.
Bryan, J.E., M.T. Jackson & N.J. Melendez, 1981. Rapid multiplication techniques for potatoes. International Potato Centre, Lma, Peru.
Capo, B.G., G. Samuels, P. Lanrau, S. Alers & A. Riera, 1955. The method of foliar diagnosis as aplied to sugar cane. Agricultural Experimental Station, University of Puerto Rico, Bulletin 123.
Debergh, P.C., 1983. Effects of agar brand and concentration on the tissue culture medium.Physiologia Plantarum 59: 270–276.
Dougall, D.K., 1981. Media factors affecting growth.Experimental Botany 21: 277–280.
Hammerschlag, F., 1982. Factors influencing in vitro multiplication and rooting of the plum rootstock myrabalan (Prunus cerasífera Ehrh.).Journal of American Society of Horticultural Science 107: 44–47.
Hussey, G. & N.J. Stacey, 1981. In vitro propagation of potato (Solanum tuberosum L.).Annals of Botany 48: 787–796.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobaco tissue cultures.Physiologia Plantarum 15: 473–497.
Pereyra, S.M. & V.S. Trippi, 1992. Las relaciones C/N y PS/N durante el crecimiento y senescencia de hojas dePhaseolus vulgaris L.Agriscientia VII: 3–7.
Pierik, R.L.M., 1990. Cultivo in vitro de las plantas superiores. Mundi-Prensa, Madrid, pp. 49–84.
Romberger, J.A. & C. Tabor, 1971. The Picea abies shoot apical meristem in culture. I. Agar and autoclaving effects.American Jounal of Botany 58: 131–140.
Rossel, G., G. De Bertoldi & R. Tizzio, 1987. In vitro mass tuberisation as a contribution to potato micropropagation.Potato Research 30: 111–116.
Singha, S., 1982. Influence of agar concentration on in vitro shoot proliferation ofMalus sp. Almey andPyrus communis Seckel.Journal of American Society of Horticultural Science 107: 657–660.
Tran Thanh Van, K. & H. Trinh, 1978. Plant microporopagation: nonidentical and identical copies. In: K.W. Hughes, R. Henke & M. Constantin (Eds.). Propagation of higher plants through tissue culture. Technological Information Center, United States Department of Energy, USA, pp. 134–158.
Tovar, P., R. Estrada, L. Schilde-Rentschler & J.H. Dodds, 1985. Inducción y utilización de tubérculos in vitro de papa. CIP-Circular 13, pp. 12.
Wetherell, D.F. & D.K. Dougall, 1976. Sources of nitrogen supporting growth and embryogenesis in cultured wild carrot tissue.Physiologia Plantarum 37: 97–103.
Yemm, E.W. & A.J. Willis. 1954. The estimation of carbohydrates in plant extracts by anthrone.Biochemistry Journal 57: 508–514.
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Del Avila, A., Pereyra, S.M. & Argüello, J.A. Potato micropropagation: Growth of cultivars in solid and liquid media. Potato Res 39, 253–258 (1996). https://doi.org/10.1007/BF02360916
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DOI: https://doi.org/10.1007/BF02360916