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Amylolytic activity in stored potato tubers. 2. The effect of low-temperature storage on the activities of α-and β-amylase and α-glucosidase in potato tubers

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Summary

Tubers of the potato cultivars Record, Wilja, Pentland Dell and Brodick (formerly clone 137371) were sampled before and after storage at either 4°C or 10°C. Reducing sugar content stayed constant during storage at 10°C in all four cultivars but rose greatly during the first 6–12 weeks of storage at 4°C in Record, Wilja and Pentland Dell but not in Brodick. Amylolytic activity was determined after 5 weeks storage using blockedp-nitrophenyl maltoheptaoside as substrate for α-amylase,p-nitrophenyl maltopentaoside as substrate for β-amylase, andp-nitrophenylglucopyranoside as substrate for α-glucosidase. The values obtained from tubers stored at 4°C were higher than those from tubers stored at 10°C, the differences being much less in Brodick than in the other three cultivars.

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References

  • ap Rees, T., W. L., Dixon, C. J. Pollock & F. Franks, 1981. Low temperature sweetening of higher plants. In: J. Friend & M. J. C. Rhodes (Eds), Recent Advances in the Biochemistry of Fruits and Vegetables. Academic Press, London. pp. 41–61.

    Google Scholar 

  • ap Rees, T., M. M. burrell, T. G. Entwistle, J. B. W. Hammond, D. Kirk & N. J. Kruger, 1988. Effects of low temperature on the respiratory metabolism of carbohydrates by plants. In: S. P. Long & F. I. Woodward (Eds), Plants and Temperature. The Company of Biologists Ltd, Cambridge. pp. 377–393.

    Google Scholar 

  • Bernfeld, P., 1955. Amylases, α and β. In: S. P. Colowick & W. D. Kaplan (Eds.), Methods in Enzymology, Vol. 1, Academic Press, New York, p 149–158.

    Google Scholar 

  • Bielinska-Czarnecka, M. & K. Bialek, 1977. Amylase and acid phosphatase activity in potato tubers treated with gibberellic acid and stored at 2 °C and 8 °C.Acta Agrobotanica 30: 95–101.

    CAS  Google Scholar 

  • Brown, J., G. R. Mackay, H. Bain, D. W. Griffith & M. J. Allison, 1990. The processing potential of tubers of the cultivated potato,Solanum tuberosum L., after storage at low temperatures. 2. Sugar concentration.Potato Research 33: 219–227.

    Google Scholar 

  • Cochrane, M. P., C. M. Duffus, M. J. Allison & G. R. Mackay, 1991. Amylolytic activity in stored potato tubers. 1. Estimation usingp-nitrophenyloligosaccharides.Potato Research 34: 325–332.

    CAS  Google Scholar 

  • Greenwood, C. T. & E. A. Milne, 1968. Starch degrading and synthesising enzymes: a discussion of their properties and action pattern.Advances in Carbohydrate Chemistry 23: 281–366.

    CAS  Google Scholar 

  • Isherwood, F. A., 1973. Starch-sugar interconversion inSolanum tuberosum.Phytochemistry 12: 2579–2591.

    Article  CAS  Google Scholar 

  • McCleary, B. V. & R. Codd, 1989. Measurement of β-amylase in cereal flours and commercial enzyme preparations.Journal of Cereal Science 9: 17–34.

    CAS  Google Scholar 

  • McCleary, B. V. & H. Sheehan, 1987. Measurement of cereal α-amylase: A new assay procedure.Journal of Cereal Science 6: 237–251.

    CAS  Google Scholar 

  • Mackay, G. R., J. Brown & C. J. W. Torrance, 1990. Processing potential of tubers of the cultivated potato,Solanum tuberosum L. after storage at low temperature. 1. Fry colour.Potato Research 33: 211–218.

    Google Scholar 

  • Müller-Thurgau, H., 1882. Sugar accumulation in portions of plants at low temperature.Landwirtschaftliche Jahrbücher 11: 751–828.

    Google Scholar 

  • Nowak, J., 1977. Biochemical changes in stored potato tubers with different rest periods. II. Influence of the storage temperature and isopropyl phenylcarbamates on enzyme activities.Zeitschrift für Pflanzenphysiologie 81: 125–140.

    CAS  Google Scholar 

  • Pollock, C. J. & T. ap Rees, 1975. Activities of enzymes of sugar metabolism in cold-stored tubers ofSolanum tuberosum.Phytochemistry 14: 613–617.

    CAS  Google Scholar 

  • Pressey, R. & R. Shaw, 1966. Effect of temperature on invertase, invertase inhibitor and sugars in potato tubers.Plant Physiology 41: 1657–1661.

    CAS  Google Scholar 

  • Richardson, D. L., H. V. Davies, H. A. Ross & G. R. Mackay, 1990. Invertase activity and its relation to hexose accumulation in potato tubers.Journal of Experimental Botany 41: 95–99.

    CAS  Google Scholar 

  • Samotus, B. & M. Leja, 1973. The extraction of amylolytic enzymes from potato tuber tissue and the determination of their activity.Ziemniak (1973): 21–29.

    Google Scholar 

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Patricia Cochrane, M., Duffus, C.M., Allison, M.J. et al. Amylolytic activity in stored potato tubers. 2. The effect of low-temperature storage on the activities of α-and β-amylase and α-glucosidase in potato tubers. Potato Res 34, 333–341 (1991). https://doi.org/10.1007/BF02360507

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