Summary
Tubers of the potato cultivars Record, Wilja, Pentland Dell and Brodick (formerly clone 137371) were sampled before and after storage at either 4°C or 10°C. Reducing sugar content stayed constant during storage at 10°C in all four cultivars but rose greatly during the first 6–12 weeks of storage at 4°C in Record, Wilja and Pentland Dell but not in Brodick. Amylolytic activity was determined after 5 weeks storage using blockedp-nitrophenyl maltoheptaoside as substrate for α-amylase,p-nitrophenyl maltopentaoside as substrate for β-amylase, andp-nitrophenylglucopyranoside as substrate for α-glucosidase. The values obtained from tubers stored at 4°C were higher than those from tubers stored at 10°C, the differences being much less in Brodick than in the other three cultivars.
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References
ap Rees, T., W. L., Dixon, C. J. Pollock & F. Franks, 1981. Low temperature sweetening of higher plants. In: J. Friend & M. J. C. Rhodes (Eds), Recent Advances in the Biochemistry of Fruits and Vegetables. Academic Press, London. pp. 41–61.
ap Rees, T., M. M. burrell, T. G. Entwistle, J. B. W. Hammond, D. Kirk & N. J. Kruger, 1988. Effects of low temperature on the respiratory metabolism of carbohydrates by plants. In: S. P. Long & F. I. Woodward (Eds), Plants and Temperature. The Company of Biologists Ltd, Cambridge. pp. 377–393.
Bernfeld, P., 1955. Amylases, α and β. In: S. P. Colowick & W. D. Kaplan (Eds.), Methods in Enzymology, Vol. 1, Academic Press, New York, p 149–158.
Bielinska-Czarnecka, M. & K. Bialek, 1977. Amylase and acid phosphatase activity in potato tubers treated with gibberellic acid and stored at 2 °C and 8 °C.Acta Agrobotanica 30: 95–101.
Brown, J., G. R. Mackay, H. Bain, D. W. Griffith & M. J. Allison, 1990. The processing potential of tubers of the cultivated potato,Solanum tuberosum L., after storage at low temperatures. 2. Sugar concentration.Potato Research 33: 219–227.
Cochrane, M. P., C. M. Duffus, M. J. Allison & G. R. Mackay, 1991. Amylolytic activity in stored potato tubers. 1. Estimation usingp-nitrophenyloligosaccharides.Potato Research 34: 325–332.
Greenwood, C. T. & E. A. Milne, 1968. Starch degrading and synthesising enzymes: a discussion of their properties and action pattern.Advances in Carbohydrate Chemistry 23: 281–366.
Isherwood, F. A., 1973. Starch-sugar interconversion inSolanum tuberosum.Phytochemistry 12: 2579–2591.
McCleary, B. V. & R. Codd, 1989. Measurement of β-amylase in cereal flours and commercial enzyme preparations.Journal of Cereal Science 9: 17–34.
McCleary, B. V. & H. Sheehan, 1987. Measurement of cereal α-amylase: A new assay procedure.Journal of Cereal Science 6: 237–251.
Mackay, G. R., J. Brown & C. J. W. Torrance, 1990. Processing potential of tubers of the cultivated potato,Solanum tuberosum L. after storage at low temperature. 1. Fry colour.Potato Research 33: 211–218.
Müller-Thurgau, H., 1882. Sugar accumulation in portions of plants at low temperature.Landwirtschaftliche Jahrbücher 11: 751–828.
Nowak, J., 1977. Biochemical changes in stored potato tubers with different rest periods. II. Influence of the storage temperature and isopropyl phenylcarbamates on enzyme activities.Zeitschrift für Pflanzenphysiologie 81: 125–140.
Pollock, C. J. & T. ap Rees, 1975. Activities of enzymes of sugar metabolism in cold-stored tubers ofSolanum tuberosum.Phytochemistry 14: 613–617.
Pressey, R. & R. Shaw, 1966. Effect of temperature on invertase, invertase inhibitor and sugars in potato tubers.Plant Physiology 41: 1657–1661.
Richardson, D. L., H. V. Davies, H. A. Ross & G. R. Mackay, 1990. Invertase activity and its relation to hexose accumulation in potato tubers.Journal of Experimental Botany 41: 95–99.
Samotus, B. & M. Leja, 1973. The extraction of amylolytic enzymes from potato tuber tissue and the determination of their activity.Ziemniak (1973): 21–29.
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Patricia Cochrane, M., Duffus, C.M., Allison, M.J. et al. Amylolytic activity in stored potato tubers. 2. The effect of low-temperature storage on the activities of α-and β-amylase and α-glucosidase in potato tubers. Potato Res 34, 333–341 (1991). https://doi.org/10.1007/BF02360507
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DOI: https://doi.org/10.1007/BF02360507