Abstract
It was demonstrated in experiments on rats that the injection of the neuropeptide, galanin (200, 500, and 1000 ng), into the lateral cerebral ventricles induced a dose-dependant decrease in the number of successful attempts at avoidance in rats trained preliminarily to active avoidance by jumping. The preliminary administration of the cholinolytic, atropine (1 mg/kg, intraperitoneally), to the rats also caused an acceleration of the damping of the conditioned reflex and potentiated the indicated effect of galanin during the experiment. The use of the opioid antagonist, naloxone (1 mg/kg, intraperitoneally), did not exert an influence on the animals' behavior, but blocked the galanin-provoked acceleration of the extinction of the active avoidance habit. The intraperitoneal administration of a noncompetitive antagonist of excitatory amino acids, ketamine (10 mg/kg), did not influence the character of the animals' behavior nor the indicated effects of galanin. It was concluded that galanin possesses an amnestic action in the active avoidance test, and that this effect of the peptide is determined by the suppression of cholinergic and activation of opiatergic transmission in the central nervous system.
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References
R. T. Bartus, R. L. Dean, B. Beer, and A. S. Lippa, “The cholinergic hypothesis of geriatric memory dysfunctions,Science,217, No. 5, 408–414 (1982).
J. Bures, J. Buresova, and J. Huston,Techniques and Basic Experiments for the Study of the Brain and Behavior, Amsterdam; New York (1983).
J. N. Crawely and G. L. Wenk, “Coexistence of galanin and acetylcholine: is galanin involved in memory processes and dementia,”Trends Neurosci.,12, No. 8, 278–282 (1989).
P. Dutar, Y. Lamour, and R. A. Nicoli, “Galanin blocks the slow cholinergic EPSP in CA-1 pyramidal neurons from ventral hippocampus,”Eur. J. Pharmacol.,164, No. 3, 583–586 (1989).
G. Fisone, C. F. Wu, S. Consolo, O. Nordstrom, et al., “Galanin inhibits acetylcholine release in the ventral hippocampus of the rat: histochemical, autoradiographic, in vivo and in vitro studies,”Proc. Natl. Acad. Sci. USA,84, No. 20, 7339–7343 (1987).
J. E. Fox, T. J. McDonald, F. Kostolanska, and K. Tatemoto, “Galanin: an inhibitory neural peptide of the canine small intestine,”Life Sci.,39, No. 2, 102–110 (1986).
Galanin: A New Multifunctional Peptide in the Neuroendocrine System, T. Hokfelt, T. Bartfai, D. Jacowitz, and D. Otteson (eds.), New York (1991).
B. S. Givens, D. S. Olton, and J. N. Crawely, “Galanin in the medial septal area impairs working memory,”Brain Res.,582, No. 1, 71–77 (1992).
P. Hedlund, G. Voneuler, and K. Fuxe, “Activation of 5-HTIA receptors increases the affinity of galanin receptors in diencephalic and telencephalic areas of the rat,”Brain Res.,560, No. 1-2, 251–259 (1991).
T. Land, U. Langel, G. Fisone, et al., “Assay for galanin receptors,” in:Methods In Neurosci., Vol. 5. Neuropeptide Technology: Gene Expression and Neuropeptide Receptors, P. M. Lenn (ed.), San-Diego (1991), pp. 225–234.
D. H. Malin, B. J. Novy, A. E. Lett-brown, et al., “Galanin attenuates retention of one-trial reward learning,”Life Sci.,50, No. 13, 939–944 (1992).
J. Mastropaolo, N. S. Madi, N. C. Ostrowsky, and J. N. Crawley, “Galanin antagonizes acetylcholine on memory task in basal forebrain-lesioned rats,”Proc. Nat. Acad. Sci. USA,87, No. 17, 6597–6560 [sic] (1989).
S. R. Michener, L. D. Aimone, T. L. Yaksh, and V. L. W. Go, “Distribution of galanin-like immunoreactivity in the pig, rat and human central nervous system,”Peptides,11, No. 6, 1217–1223 (1990).
M. Nishirobi, “Galanin inhibits noradrenaline-induced accumulation of cyclic AMP in the rat cerebral cortex,”J. Neurochem.,51, No. 6, 1953–1955 (1988).
O. Nordstrom, T. Melander, T. Hokfelt, et al., “Evidence for an inhibitory effect of the peptide galanin on dopamine release from the rat median eminence,”Neurosci. Lett.,75, No. 1, 21–26 (1987).
S. O. Ogren and A. Pramanik, “Galanin: regulation of cholinergic function and behavior,” in:Cholinergic Basis for Alzheimer Therapy, R. E. Becker and E. Giacobini (eds.), Boston (1991), pp. 193–199.
G. Paxinos and Ch. Watson,The Rat Brain in Stereotaxic Coordinates, Sydney (1982).
E. Sundstrom, T. Archer, T. Melander, and T. Hokfelt, “Galanin impairs acquisition but not retrieval of spatial memory in rats studied in the Morris swim maze,”Neurosci. Lett.,88, No. 3, 331–335 (1988).
K. Tatemoto, A. Rokaens, H. Jorvall, et al., “Galanin—a novel biologically active peptide from porcine intestine,”FEBS Lett.,164, No. 1, 124–128 (1983).
Z. Wiesenfeld-Hallin, X. J. Xu, M. J. Villar, and T. Hokfelt, “Intrathecal galanin potentiates the spinal-analgesic effect of morphine: electrophysiological and behavioral studies,”Neurosci. Lett.,109, No. 1-2, 217–221 (1990).
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Shandra, A.A., Mazarati, A.M. & Servetskii, K.L. Influence of the neuropeptide galanin on active avoidance in rats. Neurosci Behav Physiol 24, 429–432 (1994). https://doi.org/10.1007/BF02359796
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DOI: https://doi.org/10.1007/BF02359796