Study of the activity of head ganglion cells of larvae of theDrosophila ts-mutant with altered capacity for learning and increased activational properties of calmodulin
- 11 Downloads
The mitotic activity of cells of the head neural ganglion ofDrosophila larvae of two genetic lines, the agts 3-mutant line, which possesses increased calmodulin activational properties and altered capacity for learning, and the wild type CS line, serving as a control, was studied. The value of the mitotic index, as a ratio of the number of dividing cells to their total number, was assessed. The mitotic index was calculated following the exposure of the larvae to a temperature of 37°C for 30 min, and without exposure, at a temperature which was standard for the maintenance ofDrosophila ts-mutants, 22°C. A higher mitotic index was observed at 22°C in the agts 3 line as compared with the CS line. Exposure to a temperature of 37°C led to a sharp decrease in mitotic activity in both of the lines investigated. The increase in mitotic index at 22°C in the agts 3 line was presumptively related to an increase in the activational properties of calmodulin, which is characteristic for this line. Following preliminary treatment of the neural ganglia by the calmodulin inhibitor, trifluoperazine, at a concentration of 10−3 M for 30 min, the difference between the mitotic index of the mutant and the control line disappeared due to its approximately three-fold decrease in the agts 3 line; this confirmed the hypothesis advanced and suggested an important role of calmodulin in the regulation of the mitotic activity of cells of the neural ganglion ofDrosophila larvae.
KeywordsGanglion Cell Sharp Decrease Mitotic Activity Mitotic Index Control Line
Unable to display preview. Download preview PDF.
- 2.N. G. Lopatina and V. V. Ponomarenko, “The genetics of higher nervous activity,” in: A Manual on Physiology [in Russian], Nauka, Leningrad (1987), p. 9.Google Scholar
- 3.A. V. Medvedeva and E. V. Savvateeva. “The influence of ts-mutations of the agnostic gene which controls the functions of calmodulin and the capacity for learning, on the ectopic conjugation of polytene chromosomes inDrosophila,” Doklady Akad. Nauk SSSR,318. No. 3, 733 (1991).Google Scholar
- 4.V. V. Ponomarenko, “Some molecular and systemic aspects of the genetic control of behavior,” in: Works of the XI Congress of the I. P. Pavlov All-Union Physiological Society [in Russian], Leningrad (1970), p. 97.Google Scholar
- 5.N. V. Popova, The Functional Significance of Genetic Variations of Brain Weight in the House Mouse [in Russian], Moscow (1983).Google Scholar
- 6.E. V. Savvateeva, The Genetic Control of the Metabolism of Cyclic Nucleotides inDrosophila melanogaster [in Russian], Leningrad (1989).Google Scholar
- 7.E. V. Savvateeva, “The genetic control of systems of second messengers and their role in learning,” in: Advances in Contemporary Genetics, Vol. 17 [in Russian], Nauka, Moscow (1991), p. 33.Google Scholar
- 9.Z. Asztalos, J. Wegerer, G. Wustmann, et al., “Protein phosphatase i-deficient mutantDrosophila is affected in habituation and associative learning,” J. Neuroscience,13, 924 (1993).Google Scholar
- 12.H. J. Berridge, “Control of cell division: a unifying hypothesis,” J. Cycl. Nucl. Res.,1, 305 (1975).Google Scholar
- 16.M. Gatti, C. Tansarella, and G. Olivieri, “Analysis of the chromosome aberrations induced by x-rays in somatic cells ofDrosophila,” Genetics,77, 701.Google Scholar
- 20.A. Pardee, R. Dubrow, J. Hamlin, and R. Kleitzen, “Animal cell cycle,” Ann. Rev. Biochem.,47, 751 (1978).Google Scholar
- 24.W. J. Vasserman and L. D. Smith, “Calmodulin triggers the resumption of mitosis in amphibian oocytes,” J. Cell Biol.,89, 389 (1981).Google Scholar