Advertisement

Neuroscience and Behavioral Physiology

, Volume 26, Issue 5, pp 435–440 | Cite as

Study of the activity of head ganglion cells of larvae of theDrosophila ts-mutant with altered capacity for learning and increased activational properties of calmodulin

  • E. V. Tokmacheva
Article
  • 12 Downloads

Abstract

The mitotic activity of cells of the head neural ganglion ofDrosophila larvae of two genetic lines, the agts 3-mutant line, which possesses increased calmodulin activational properties and altered capacity for learning, and the wild type CS line, serving as a control, was studied. The value of the mitotic index, as a ratio of the number of dividing cells to their total number, was assessed. The mitotic index was calculated following the exposure of the larvae to a temperature of 37°C for 30 min, and without exposure, at a temperature which was standard for the maintenance ofDrosophila ts-mutants, 22°C. A higher mitotic index was observed at 22°C in the agts 3 line as compared with the CS line. Exposure to a temperature of 37°C led to a sharp decrease in mitotic activity in both of the lines investigated. The increase in mitotic index at 22°C in the agts 3 line was presumptively related to an increase in the activational properties of calmodulin, which is characteristic for this line. Following preliminary treatment of the neural ganglia by the calmodulin inhibitor, trifluoperazine, at a concentration of 10−3 M for 30 min, the difference between the mitotic index of the mutant and the control line disappeared due to its approximately three-fold decrease in the agts 3 line; this confirmed the hypothesis advanced and suggested an important role of calmodulin in the regulation of the mitotic activity of cells of the neural ganglion ofDrosophila larvae.

Keywords

Ganglion Cell Sharp Decrease Mitotic Activity Mitotic Index Control Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    M. A. Gofman, N. I. Dmitrieva, and N. G. Lopatina, “The density of neurons in different regions of the brain of mice with trisomy and translocation in the T6 autosome.” Fiziol. Zh. SSSR,66, 594 (1980).PubMedGoogle Scholar
  2. 2.
    N. G. Lopatina and V. V. Ponomarenko, “The genetics of higher nervous activity,” in: A Manual on Physiology [in Russian], Nauka, Leningrad (1987), p. 9.Google Scholar
  3. 3.
    A. V. Medvedeva and E. V. Savvateeva. “The influence of ts-mutations of the agnostic gene which controls the functions of calmodulin and the capacity for learning, on the ectopic conjugation of polytene chromosomes inDrosophila,” Doklady Akad. Nauk SSSR,318. No. 3, 733 (1991).Google Scholar
  4. 4.
    V. V. Ponomarenko, “Some molecular and systemic aspects of the genetic control of behavior,” in: Works of the XI Congress of the I. P. Pavlov All-Union Physiological Society [in Russian], Leningrad (1970), p. 97.Google Scholar
  5. 5.
    N. V. Popova, The Functional Significance of Genetic Variations of Brain Weight in the House Mouse [in Russian], Moscow (1983).Google Scholar
  6. 6.
    E. V. Savvateeva, The Genetic Control of the Metabolism of Cyclic Nucleotides inDrosophila melanogaster [in Russian], Leningrad (1989).Google Scholar
  7. 7.
    E. V. Savvateeva, “The genetic control of systems of second messengers and their role in learning,” in: Advances in Contemporary Genetics, Vol. 17 [in Russian], Nauka, Moscow (1991), p. 33.Google Scholar
  8. 8.
    D. Allen, J. Munshower, H. Morris, and G. Weter, “Regulation of adenylcyclase in hepatomas of different growth rates,” Cancer Res.,31, 557 (1971).PubMedGoogle Scholar
  9. 9.
    Z. Asztalos, J. Wegerer, G. Wustmann, et al., “Protein phosphatase i-deficient mutantDrosophila is affected in habituation and associative learning,” J. Neuroscience,13, 924 (1993).Google Scholar
  10. 10.
    A. Balling, G. Technau, and M. Heisenberg, “Are the structural changes in adultDrosophila mushroom bodies memory traces? Studies on biochemical learning mutants,” J. Neurogenet.,4, 65 (1987).PubMedGoogle Scholar
  11. 11.
    M. Barrau, G. Blackburn, and W. Dewey, “Effect of heat shock on the centrosomes of Chinese hamster ovary cells,” Cancer Res.,38, 2290 (1978).PubMedGoogle Scholar
  12. 12.
    H. J. Berridge, “Control of cell division: a unifying hypothesis,” J. Cycl. Nucl. Res.,1, 305 (1975).Google Scholar
  13. 13.
    J. G. Chafouleas, W. E. Bolton, H. Hidaka, et al., “Calmodulin and the cell cycle involvement in regulation of cell cycle progression,” Cell,28, 41 (1982).CrossRefPubMedGoogle Scholar
  14. 14.
    W. Y. Cheung, “Calmodulin plays a pivotal role in cell regulation,” Science,207, 19 (1980).PubMedGoogle Scholar
  15. 15.
    J. C. Eccles, “Calcium in long-term potentiation as a model for memory,” Neuroscience,10, 1071 (1983).CrossRefPubMedGoogle Scholar
  16. 16.
    M. Gatti, C. Tansarella, and G. Olivieri, “Analysis of the chromosome aberrations induced by x-rays in somatic cells ofDrosophila,” Genetics,77, 701.Google Scholar
  17. 17.
    M. Heisenberg, A. Borst, S. Wagner, and D. Byers, “Drosophila mushroom body mutants are deficient in olfactory learning,” J. Neurogenet.,2, 1 (1985).PubMedGoogle Scholar
  18. 18.
    A. Means and J. Dedman, “Calmodulin—an intracellular calcium receptor,” Nature,285, 73 (1980).CrossRefPubMedGoogle Scholar
  19. 19.
    R. L. Ney, N. Hochella, et al., “Abnormal regulation of adenosime 3′5-monophosphate and corticosterone formation in an adrenocortical carcinoma,” J. Clin. Investig.,48, 1733 (1969).PubMedGoogle Scholar
  20. 20.
    A. Pardee, R. Dubrow, J. Hamlin, and R. Kleitzen, “Animal cell cycle,” Ann. Rev. Biochem.,47, 751 (1978).Google Scholar
  21. 21.
    I. Rensing and R. Hardeland, “Effects of cyclic adenosine 3′5-monophosphate on membrane potential, nuclear volume and puff size inDrosophila salivary glandsin vitro,” Exp. Cell. Res.,73, 311 (1972).CrossRefPubMedGoogle Scholar
  22. 22.
    T. Tanaka and H. Hidaka, “Hydrophobic regions function in calmodulin-enzyme(s) interactions,” J. Biol. Chem.,255, 11078 (1980).PubMedGoogle Scholar
  23. 23.
    A. Tissieres, H. K. Mitchell, and A. M. Tracy, “Protein synthesis in salivary glands ofDrosophila melanogaster: relation to chromosome puffs,” J. Mol. Biol.,84, 389 (1974).CrossRefPubMedGoogle Scholar
  24. 24.
    W. J. Vasserman and L. D. Smith, “Calmodulin triggers the resumption of mitosis in amphibian oocytes,” J. Cell Biol.,89, 389 (1981).Google Scholar
  25. 25.
    C. Wimer, P. Wimer, and J. S. Wimer, “An association between granule cell density in the dentate gyrus and two-way avoidance conditioning in the house mouse.” Behav. Neurosci.,97, 844 (1983).CrossRefPubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • E. V. Tokmacheva

There are no affiliations available

Personalised recommendations