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Potato Research

, Volume 33, Issue 3, pp 365–375 | Cite as

Preparation of additional monoclonal antibodies for detection and discrimination of potato virus Y isolates infecting potato

  • A. Sanz
  • M. Cambra
  • C.Perez De San Roman
  • J. G. Miguet
  • E. Cortes
  • M. T. Gorris
  • C. Vela
Article

Summary

Monoclonal antibodies (MAb) specific to potato virus Y (PVY) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate Bintje PVYo. Six MAb were characterized. The reactions of the MAb have been assayed by indirect double-antibody sandwich enzyme-linked immunosorbent assay (ELISA-IDAS) against forty-nine PVY isolates and other potyviruses, representative of different potato areas in the world.

All MAb reacted to at least some isolates in group O+C in ELISA-IDAS. One MAb did not react to any group N isolates. MAb 10E3 reacted to all isolates tested. Using standard double-antibody sandwich enzyme-linked immunosorbent assay (ELISA-DAS), two MAbs did not react to any isolates, one reacted to some isolates in group O+C and MAb 10E3 reacted to all isolates but two in group C. A mixture of MAb 10E3 and 1E10 detected all isolates in ELISA-DAS and sensitivity was improved over that obtained with polyclonal antibodies from antisera.

Additional key words

ELISA serotyping pepper 

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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • A. Sanz
    • 1
  • M. Cambra
    • 2
  • C.Perez De San Roman
    • 3
  • J. G. Miguet
    • 1
  • E. Cortes
    • 1
  • M. T. Gorris
    • 2
  • C. Vela
    • 1
  1. 1.Immunología y Genética Aplicada S.A. (INGENASA)MadridSpain
  2. 2.Instituto Valenciano de Investigaciones Agrarias (IVIA)Moncada ValenciaSpain
  3. 3.Estación de Mejora de la Patata, Gobierno VascoVitoria-GasteizSpain

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