Skip to main content

Advertisement

SpringerLink
Log in

Detection of anti-double and anti-single stranded DNA antibodies in chronic liver disease: Significance of anti-double stranded DNA antibody in autoimmune hepatitis

  • Liver, Pancreas, and Biliary Tract
  • Published:
Journal of Gastroenterology Aims and scope Submit manuscript

Abstract

Anti-DNA antibodies were determined by an enzyme-linked immunosorbent assay in 116 patients with chronic liver disease consisting of 21 cases of autoimmune hepatitis (AIH), 17 of primary biliary cirrhosis (PBC), and 78 of non-autoimmune-type of chronic liver disease. The assay was also performed on 83 patients with collagen disease, as a control group. Anti-double stranded DNA antibody (anti-dsDNA) was detected in 10/21 (48%) of the AIH patients and in 3/17 (17%) of the PBC patients, but not in those with other liver diseases. In contrast, anti-single stranded DNA antibody (anti-ssDNA) was positive not only in AIH and PBC, but also in those with non-autoimmune-types of chronic liver disease. Follow-up liver histology disclosed that the 2 patients with AIH who were positive for anti-dsDNA developed liver cirrhosis, whereas the 4 patients who were negative for anti-dsDNA, and those who showed a disappearance of anti-dsDNA following corticosteroid therapy, improved from chronic active to chronic persistent hepatitis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sontheimer RD, Gilliam JN. An immunofluorescence assay for double-stranded DNA antibodies using theCrithidia luciliae kinetoplast as a double-stranded DNA substrate. J Lab Clin Med 1978;91:550–558.

    PubMed  CAS  Google Scholar 

  2. Eaton RB, Schnneider G, Schur PH. Enzyme immunoassay for antibodies to native DNA. Arthritis Rheum 1983;26:52–62.

    PubMed  CAS  Google Scholar 

  3. Jain S, Markham R, Thomas HC, et al. Double-stranded DNA-binding capacity of serum in acute and chronic liver disease. Clin Exp Immunol 1976;26:35–41.

    PubMed  CAS  Google Scholar 

  4. Kingham JGC, Rassam S, Ganguly NK, et al. DNA-binding antibodies and hepatitis B markers in acute and chronic liver disease. Clin Exp Immunol 1978;33:204–210.

    PubMed  CAS  Google Scholar 

  5. Davis P, Read AE. Antibodies to double-stranded (native) DNA in active chronic hepatitis. Gut 1975;16:413–415.

    PubMed  CAS  Google Scholar 

  6. Gurian LE, Rogoff TM, Ware AJ, et al. The immunologic diagnosis of chronic active “autoimmune” hepatitis: Distinction from systemic lupus erythematosus. Hepatology 1985;5:397–402.

    PubMed  CAS  Google Scholar 

  7. Leggett BA, Collins RJ, Cooksley WGE, et al. Evaluation of the Crithidia assay to distinguish between auto-immune chronic active hepatitis and systemic lupus erythematosus. J Gastroenterol Hepatol 1987;2:205–211.

    Google Scholar 

  8. Wood JR, Czaja AJ, Beaver SJ, et al. Frequency and significance of antibody to double-stranded DNA in chronic active hepatitis. Hepatology 1986;6:976–980.

    PubMed  CAS  Google Scholar 

  9. Mackay IR, Weiden S, Hasker J. Autoimmune hepatitis. Ann N Y Acad Scie 1965;124:767–780.

    CAS  Google Scholar 

  10. Tan EM, Cohen AS, Fries JF, et al. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1982;25:1271–1277.

    PubMed  CAS  Google Scholar 

  11. Arnett FC, Edworthy SM, Bloch DA, et al. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum 1988;31:315–324.

    PubMed  CAS  Google Scholar 

  12. Subcommittee for Scleroderma Criteria of the American Rheumatism Association Diagnostic and Therapeutic Criteria Committee. Preliminary criteria for the classification of systemic sclerosis (scleroderma). Arthritis Rheum 1980;23:581–590.

    Google Scholar 

  13. Fox RI, Robinson CA, Curd JG, et al. Sjögren's syndrome. Proposed criteria for classification. Arthritis Rheum 1986;29:577–585.

    PubMed  CAS  Google Scholar 

  14. Bohan A, Peter JB. Polymyositis and dermatomyositis (first of two parts). N Engl J Med 1975;292:344–347.

    PubMed  CAS  Google Scholar 

  15. Sharp GC, Irvin WS, Tan EM, et al. Mixed connective tissue disease—an apparently distinct rheumatic disease syndrome associated with a specific antibody to an extractable nuclear antigen (ENA). Am J Med 1972;52:148–159.

    Article  PubMed  CAS  Google Scholar 

  16. Locker JD, Medof ME, Bennet RM, et al. Characterization of DNA used to assay sera for anti-DNA antibodies; determination of the specificities of anti-DNA antibodies in SLE and non-SLE rheumatic disease status. J Immunol 1977;118:694–701.

    PubMed  CAS  Google Scholar 

  17. Arana R, Seligmann M. Antibodies to native and denatured deoxyribonucleic acid in systemic lupus erythenatosus. J Clin Invest 1967;46:1867–1882.

    PubMed  CAS  Google Scholar 

  18. Koike T, Tomioka H, Kumagai A. Antibodies cross-reactive with DNA and cardiolipin in patients with systemic lupus erythematosus. Clin Exp Immunol 1982;50:298–302.

    PubMed  CAS  Google Scholar 

  19. Faaber P, Capel PJA, Rijke GPM, et al. Cross-reactivity of anti-DNA antibodies with proteoglycans. Clin Exp Immunol 1984;55:502–508.

    PubMed  CAS  Google Scholar 

  20. Villarejos VM, Arqembourg PC, Visoná KA, et al. Antibodies to single-stranded DNA: An aid in diagnosis of viral hepatitis. J Med Virol 1978;2:359–367.

    PubMed  CAS  Google Scholar 

  21. Villarejos VM, Serra J, Visoná KA, et al. Antibodies to single stranded DNA: A diagnostic aid in chronic hepatitis B virus infections. J Med Virol 1979;4:97–101.

    PubMed  CAS  Google Scholar 

  22. Somerfield SD, Roberts MW, Booth RJ. Double-stranded DNA antibodies: A comparison of four methods of detection. J Clin Pathol 1981;34:1032–1035.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Second Department of Internal Medicine, Shinshu University School of Medicine, 3-1-1, Asahi, Matsumoto, 390, Nagano, Japan

    Kimiaki Tsuchiya, Kendo Kiyosawa, Haruhiko Imai, Takeshi Sodeyama & Seiichi Furuta

Authors
  1. Kimiaki Tsuchiya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kendo Kiyosawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Haruhiko Imai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Takeshi Sodeyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Seiichi Furuta
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tsuchiya, K., Kiyosawa, K., Imai, H. et al. Detection of anti-double and anti-single stranded DNA antibodies in chronic liver disease: Significance of anti-double stranded DNA antibody in autoimmune hepatitis. J Gastroenterol 29, 152–158 (1994). https://doi.org/10.1007/BF02358676

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02358676

Key words

Search

Navigation