Abstract
We identified and quantitated theY′ bile acid binder, i.e., 3α-hydroxysteroid dehydrogenase (3α-HSD), in rat small intestinal mucosa, and compared its longitudinal distribution with that of glutathione S-transferases (GST). The enzyme activity of 3α-HSD in intestinal mucosa was approximately one-third of that in liver, and it had similar activity in the proximal, middle, and distal portions of the intestine. Immunoreactive protein corresponding to hepatic bile acid binder was detected in all segments of rat small intestine mucosal cytosol by Western blot analysis. There was no significant difference in the concentration of bile acid binder, assayed by enzyme-linked immunosorbent assay (ELISA), between the proximal and the distal intestine, this being 2.93±0.03 and 3.29±0.95 nmol/g tissue, respectively (mean±SD of four animals). On the other hand, the concentration of GST 1-1 showed sharp longitudinal decline and that of GST 3–4 was negligible in the small intestine, as we previously reported, indicating that bile acid binder was a prominent cytosol binding protein in the distal intestine. These results suggested the possible role of bile acid binder in the intracellular transport of bile acids in the ileum.
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Carry MC, Cahalane MJ. Enterohepatic circulation. In: Arias IM, Jakoby WB, Popper H, Schachter D, Shafritz DA (eds) The liver: Biology and pathobiology. 2nd ed New York: Raven, 1988:573–641.
Sugiyama Y, Stolz A, Sugimoto M, et al. Evidence for a common high affinity binding site on glutathione S-transferase B for lithocholic acid and bilirubin. J Lipid Res 1984;25:1177–1183.
Takikawa H, Kaplowitz N. Comparison of the binding sites of GSH S-transferases of the Ya-and Yb-subunit classes: Effect of glutathione on the binding of bile acids. J Lipid Res 1988;29: 279–286.
Takikawa H, Sugiyama Y, Kaplowitz N. Binding of bile acids by glutathione S-transferases from rat liver. J Lipid Res 1986; 27:955–966.
Sugiyama Y, Yamada T, Kaplowitz N. Newly identified bile acid binders in rat liver cytosol: Purification and comparison with glutathione S-transferases. J Biol Chem 1983;258:3602–3607.
Takikawa H, Kaplowitz N. Binding of bile acids, oleic acid, and organic anions by rat and human hepatic Z protein. Arch Biochem Biophys 1986;251:385–392.
Stolz A, Takikawa H, Sugiyama Y, et al.: 3α-hydroxysteroid dehydrogenase activity of theY′ bile acid binders in rat liver cytosol: Identification, kinetics, and physiologic significance. J Clin Invest 1987;79:427–434.
Stolz A, Sugiyama Y, Kuhlenkamp J, et al. Cytosolic bile acid binding protein in rat liver: Radioimmunoassay, molecular forms, developmental characteristics, and organ distribution. Hepatology 1986;6:433–439.
Smithgall TE, Penning TM. Electrophoretic and immunochemical characterization of 3α-hydroxysteroid/dihydrodiol dehydrogenases of rat tissues. Biochem J 1988;254:715–721.
Yamamuro W, Saiki H, Sugimoto M. Distribution of glutathione S-transferase isozymes in rat small intestinal mucosa (in Japanese). Jpn J Gastroenterol 1991;88:1184–1190.
Laemmli UK. Cleavage of structural proteins during assembly of the head of bacteriophage T4. J Biol Chem 1982;257:9909–9912.
Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gel to nitrocellulose paper: Procedure and some applications. Proc Natl Acad Sci USA 1979;76:4350–4354.
Stolz A, Rahimi-Kiani M, Ameis D, et al. Molecular structure of rat hepatic 3α-hydroxyseroid dehydrogenase: A member of the oxidoreductase gene family. J Biol Chem 1991;266:15253–15257.
Tipping E, Ketterer B, Christodoulides L, et al. The noncovalent binding of small molecules by ligandin. Eur J Biochem 1976;67:583–590.
Strange RC, Cramb R, Hayes JD, et al. Partial purification of two lithocholic acid-binding proteines from rat liver 100 000g supernatants. Biochem J 1977;165:425–429.
Hayes JD, Strange RC, Percy-Robb IW, et al. Identification of two lithocholic acid-binding proteins. Biochem J 1979;181: 699–788.
Hayes JD, Strange RC, Percy-Robb IW, et al. Cholic acid binding by glutathione S-transferases from rat liver cytosol. Biochem J 1980;165:83–87.
Hayes JD, Strange RC, Percy-Robb IW, et al. A study of the structure of the YaYa and YaYe glutathione S-transferases from rat liver cytosol. Biochem J 1981;197:491–502.
Stolz A, Sugiyama Y, Kuhlenkamp J, et al. Identification and purification of a 36-kDa bile acid binder in human hepatic cytosol. FEBS Lett 1984;177:31–35.
Pattinson NR. Isolation of two cholic acid-binding proteins from rat liver cytosol using affinity chromatography. Biochem Biophys Acta 1981;667:70–76.
Maruyama H, Arias IM, Listowsky I. Distinctions between the multiple cationic forms of rat liver glutathione S-transferase. J Biol Chem 1984;259:12444–12448.
Maruyama H, Listowsky I. Preferential binding of steroids by aniomic forms of rat glutathione S-transferase. J Biol Chem 1984;259:12449–12555.
Ockner RK, Manning JA. Fatty acid-binding protein in small intestine: Identification, isolation, and evidence for its role in cellular fatty acid transport. J Clin Invest 1974;54:326–338.
Suzuki T, Hitomi M, Ono T. Immunohistochemical distribution of hepatic fatty acid-binding protein in rat and human alimentary tract. J Histochem Cytochem 1988;36:349–357.
Sacchettini JC, Hauft SM, Van Camp SL, et al. Developmental and structural studies of an intracellular lipid-binding protein expressed in the ileal epithelium. J Biol Chem 1990;265:19199–19207.
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Yamamuro, W., Stolz, A., Takikawa, H. et al. Distribution of 3α-hydroxysteroid dehydrogenase (bile acid binder) in rat small intestine: Comparison with glutathione S-transferase subunits. J Gastroenterol 29, 115–119 (1994). https://doi.org/10.1007/BF02358670
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DOI: https://doi.org/10.1007/BF02358670