Potato Research

, Volume 42, Issue 1, pp 181–188 | Cite as

Influence of cultivar, season, explant type and seaweed concentrate on potato plantlet quality

  • Britta Kowalski
  • Anna K. Jäger
  • J. Van Staden
Full Papers


The addition of a seaweed concentrate to tissue culture medium improved the quality of potato plantlets. Season, cultivar and explant type affected the most beneficial concentration applied. Concentrations higher or lower than the optimum were less beneficial or adverse to plantlet quality. Terminal buds were more sensitive to non-optimum seaweed application than axillary buds. Decreased water loss from plantlets was one of the potential benefits of seaweed concentrate in vitro, thus improving establishment after transplanting.

Additional keywords

micropropagation Solanum tuberosum


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Capellades, M., R. Fontarnau, C. Carulla & P. Debergh, 1990. Environment influences anatomy of stomata and epidermal cells in tissue-culturedRosa multiflora.Journal of the American Society of Horticultural Science 115: 141–145.Google Scholar
  2. Cassells, A.C., B. Kowalski, D.M. Fitzgerald & G.A. Murphy, 1998. Detection of epigenetic variation in micropropagated potato plants by leaf image analysis. EU COST meeting, Brussels (in press).Google Scholar
  3. Cassells, A.C. & T.D. Roche, 1994. The influence of the gas permeability of the vessel lid and growth room light intensity on the characteristics of Dianthus microplantsin vitro andex vitrum. In: P.J. Lumsden, J.R. Nicholas & W.J. Davies (Eds). Physiology, Growth and Development of Plants in Culture. Kluwer Academic Publishers, The Netherlands, p. 204–214.Google Scholar
  4. Conner, L.N. & A.J. Conner, 1984. Comparative water loss from leaves ofSolanum laciniatum plants cultured in vitro and in vivo.Plant Science Letters 36: 241–246.CrossRefGoogle Scholar
  5. Crouch, I.J. & J. Van Staden, 1991. Effect of seaweed concentrate on the growth of potatoes in vitro.South African Journal of Science 87: 275–276.Google Scholar
  6. Crouch, I.J. & J. Van Staden, 1994. Commercial seaweed products as biostimulants in horticulture.Journal of Home & Consumer Horticulture 1: 19–76.Google Scholar
  7. Crouch, I.J., M.T. Smith, J. Van Staden, M.J. Lewis & G.V. Hoad, 1992. Identification of auxins in a commercial seaweed concentrate.Journal of Plant Physiology 139: 590–594.Google Scholar
  8. Featonby-Smith, B.C. & J. Van Staden, 1984. Identification and seasonal variation of endogenous cytokinins inEcklonia maxima (Osbeck) Papenf.Botanica Marina 17: 527–531.Google Scholar
  9. George, E.F., 1996. Plant propagation by tissue culture, Part 2, In practice. 2nd revised edition. Exegetics Ltd., Edington, 1996.Google Scholar
  10. Grout, B.W.W. & M.J. Aston, 1977. Transplanting of cauliflower plants regenerated from meristem culture. I. Water loss and water transfer related to changes in leaf wax and to xylem regeneration.Horticultural Research 17: 1–7.Google Scholar
  11. Kowalski, B., A.K. Jäger & J. Van Staden, 1999. The effect of a seaweed concentrate on the in vitro growth and acclimatization of potato plantlets.Potato Research 42: 131–139.Google Scholar
  12. Murashige T. & F. Skoog, 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures.Physiologia Plantarum 15: 473–497.Google Scholar
  13. Roche, T.D., 1995. The influence of differentially permeable plastic films on the in vitro development and ex vitrum establishment of micropropagated plantlets. Ph.D. Thesis, National University of Ireland, University College, Cork, pp. 328.Google Scholar
  14. Sutter, E. & R.W. Langhans, 1979. Epicuticular wax formation on carnation plantlets regenerated from shoot tip culture.Journal of the American Society for Horticultural Science 104:493–496.Google Scholar
  15. Sutter, E. & R.W. Langhans, 1982. Formation of epicuticular was and its effect on water loss in cabbage plants regenerated from shoot tip culture.Canadian Journal of Botany 60: 2896–2902.Google Scholar
  16. Zaid, A. & H.G. Hughes, 1995. Water loss and polyethylene glycol-mediated acclimatization of in vitro-grown seedlings of 5 cultivars of date palm (Phoenix dactilyfera L.).Plant Cell Reports 14: 385–388.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Britta Kowalski
    • 1
  • Anna K. Jäger
    • 1
  • J. Van Staden
    • 1
  1. 1.Research Centre for Plant Growth and Development, School of Botany and ZoologyUniversity of Natal PietermaritzburgScottsvilleSouth Africa

Personalised recommendations