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Detection of tobacco rattle virus in potato tubers using a simple RT-PCR procedure

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Summary

A simple assay for detection of tobacco rattle virus in infected tubers involving RT-PCR was developed. The assay detected a wide range of strains, including nonparticle NM variants. RNA extraction methods were compared, and a simple method was found to give reliable results. The method enabled detection of at least 1 ng of tobacco rattle particles in 100 mg tuber extract.

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Authors and Affiliations

  1. Department of Plant Protection, Research Centre Flakkebjerg, Danish Institute of Agricultural Sciences, DK-4200, Slagelse, Denmark

    Mogens Nicolaisen & Steen Lykke Nielsen

  2. Plant Protection Institute, Pannon University of Agricultural Sciences, H8360, Keszthely, Hungary

    Zoltán Bösze

Authors
  1. Mogens Nicolaisen
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  2. Zoltán Bösze
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  3. Steen Lykke Nielsen
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Nicolaisen, M., Bösze, Z. & Nielsen, S.L. Detection of tobacco rattle virus in potato tubers using a simple RT-PCR procedure. Potato Res 42, 173–179 (1999). https://doi.org/10.1007/BF02358407

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  • DOI: https://doi.org/10.1007/BF02358407

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