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Journal of Gastroenterology

, Volume 29, Issue 3, pp 257–264 | Cite as

Expression of adhesion molecules and HLA-DR by macrophages and dendritic cells in aphthoid lesions of Crohn's disease: An immunocytochemical study

  • Kimitomo Morise
  • Takeo Yamaguchi
  • Atsuo Kuroiwa
  • Kazuhiro Kanayama
  • Toshihiro Matsuura
  • Masataka Shinoda
  • Hitoshi Yamamoto
  • Yoh Horiuchi
  • Atsushi Furusawa
  • Hiroaki Iwase
  • Kazuo Kusugami
  • Hiroshi Nagura
Alimentary Tract

Abstract

The phenotypes and ultrastructure of macrophages and dendritic cells in aphthoid lesions of the colon were immunocytochemically observed in patients with Crohn's disease. Biopsy specimens were endoscopically obtained from both aphthoid and advanced lesions in Crohn's disease patients. Biopsy specimens obtained from patients with infectious colitis and from normal individuals served as controls. Aphthoid lesions contained densely aggregated CD68+ macrophages, which were surrounded by numerous ID-1+ dendritic cells. In the normal controls and infectious colitis patients, however, a few scattered CD68+ macrophages and ID-1+ dendritic cells were noted beneath the surface epithelium. CD3+ lymphocytes were significantly increased in both aphthoid and advanced lesions of Crohn's disease, but the CD4/CD8 ratio was similar in all groups studied. The double immunoperoxidase staining method revealed that both CD68+ macrophages and ID-1+ dendritic cells in the aphthoid lesions simultaneously expressed ICAM-1 and HLA-DR antigens. Electronmicroscopic observation revealed that CD68+ macrophages had numerous vesicles and lysosomal granules and few projections, and that ID-1+ dendritic cells had appreciable cytoplasmic protrusions with a few vacuoles. These findings suggested that the colonic mucosa in Crohn's disease contained two types of macrophage/dendritic cells in the same lineage that expressed intercellular adhesion molecules and class-II MHC antigens. It also appeared that the aphthoid lesions of Crohn's disease featured an increase in macrophages and dendritic cells consistent with immunological activation.

Key words

macrophage dendritic cell aphthoid lesion Crohn's disease 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Kimitomo Morise
    • 1
  • Takeo Yamaguchi
    • 1
  • Atsuo Kuroiwa
    • 1
  • Kazuhiro Kanayama
    • 1
  • Toshihiro Matsuura
    • 1
  • Masataka Shinoda
    • 1
  • Hitoshi Yamamoto
    • 1
  • Yoh Horiuchi
    • 1
  • Atsushi Furusawa
    • 1
  • Hiroaki Iwase
    • 1
  • Kazuo Kusugami
    • 1
  • Hiroshi Nagura
    • 2
  1. 1.First Department of Internal MedicineNagoya University School of MedicineNagoyaJapan
  2. 2.Department of PathologyTohoku University School of MedicineSendaiJapan

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