Potato Research

, Volume 37, Issue 4, pp 413–421 | Cite as

Initial acytokinesis during leaf protoplast culture of dihaploid and tetraploidSolanum tuberosum and diploidS. bulbocastanum

  • W. J. Van Everdink
  • L. P. Pijnacker


Leaf protoplasts of dihaploid (2n=2x=24) and tetraploid (2n=4x=48)Solanum tuberosum, and diploidS. bulbocastanum (2n=2x=24) were cultured in liquid medium. The cultures were studied for early karyological changes during their development. Giemsa staining of spread preparations revealed extremely low percentages of protoplasts developing into calli with the parental chromosome number, and high percentages of acytokinetic cells. The nuclear divisions within a cell were synchronous which allowed the occurrence of spindle interaction, resulting in nuclear poly- and aneuploidization. Although polyploidization was also found in uninucleate cells, a major increase in the formation of true-to-type calli would certainly be established by the improvement of early cross wall formation.

Additional keywords

somaclonal variation chromosome number potato polyploidization aneuploidization 


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • W. J. Van Everdink
    • 1
  • L. P. Pijnacker
    • 1
  1. 1.Department of Genetics, Biological CentreUniversity of GroningenHarenThe Netherlands

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