Potato Research

, Volume 37, Issue 4, pp 413–421 | Cite as

Initial acytokinesis during leaf protoplast culture of dihaploid and tetraploidSolanum tuberosum and diploidS. bulbocastanum

  • W. J. Van Everdink
  • L. P. Pijnacker
Article
  • 27 Downloads

Summary

Leaf protoplasts of dihaploid (2n=2x=24) and tetraploid (2n=4x=48)Solanum tuberosum, and diploidS. bulbocastanum (2n=2x=24) were cultured in liquid medium. The cultures were studied for early karyological changes during their development. Giemsa staining of spread preparations revealed extremely low percentages of protoplasts developing into calli with the parental chromosome number, and high percentages of acytokinetic cells. The nuclear divisions within a cell were synchronous which allowed the occurrence of spindle interaction, resulting in nuclear poly- and aneuploidization. Although polyploidization was also found in uninucleate cells, a major increase in the formation of true-to-type calli would certainly be established by the improvement of early cross wall formation.

Additional keywords

somaclonal variation chromosome number potato polyploidization aneuploidization 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bokelmann, G.S. & S. Roest, 1983. Plant regeneration from protoplasts of potato (Solanum tuberosum cv. Bintje).Zeitung für Pflanzenphysiologie 109: 259–265.Google Scholar
  2. Bříza, J. & I. Machová, 1991. Regeneration of plants from leaf mesophyll protoplasts of the tetraploid potato cultivars Xenia and Bintje.Biologica Plantarum 33: 225–233.Google Scholar
  3. Cardi, T., K.J. Puite & K. Sree Ramulu, 1990. Plant regeneration from mesophyll protoplasts ofSolanum commersonii Dun.Plant Science 70: 215–221.CrossRefGoogle Scholar
  4. Carlberg, I., K. Glimelius & T. Eriksson, 1984. Nuclear DNA-content during the initiation of callus formation from isolated protoplasts ofSolanum tuberosum L.Plant Science Letters 35: 225–230.CrossRefGoogle Scholar
  5. Eriksson, T. & K. Jonasson, 1969. Nuclear division in isolated protoplasts from cells of higher plants grown in vitro.Planta 89: 85–89.CrossRefGoogle Scholar
  6. Everdink, W.J. van, 1994. Occurrence and Reduction of Acytokinesis in Leaf Protoplast Cultures of Potato and Tobacco: Implications for Chromosome Number Variation. PhD thesis, University of Groningen, The Netherlands.Google Scholar
  7. Fehér, A., J. Preiszner & D. Dudits, 1989. Differentiation of potato (Solanum tuberosum L.) plants from cultured leaf protoplasts.Acta Biologica Hungarica 40: 369–380.PubMedGoogle Scholar
  8. Fish, N. & A. Karp, 1986. Iniprovements in regeneration from protoplasts of potato and studies on chromosome stability.Theoretical and Applied Genetics 72: 405–412.CrossRefGoogle Scholar
  9. Fowke, L.C., C.W. Bech-Hansen, O.L. Gamborg & F. Constabel, 1975. Electron-microscope observations of mitosis and cytokinesis in multinucleate protoplasts of soybean.Journal of Cell Science 18: 491–507.PubMedGoogle Scholar
  10. Galbraith, D.W., T.J. Mauch & B.A. Shields, 1981. Analysis of the initial stages of plant protoplast development using 33258 Hoechst: reactivation of the cell cycle.Physiologica Plantarum 51: 380–386.Google Scholar
  11. Hahne, B. & F. Hoffmann, 1986. Cytogenetics of protoplast cultures ofBrachycome dichromosomatica andCrepis capillaris and regeneration of plants.Theoretical and Applied Genetics 72: 244–251.CrossRefGoogle Scholar
  12. Huang, H.-C. & C.-C. Chen, 1988. Genome multiplication in cultured protoplasts of twoNicotiana species.Journal of Heredity 79: 28–32.Google Scholar
  13. Jacobsen, E., 1987. Genetic diversity in protoplast- and cell-derived plants of potato. In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry, Vol. 3, Springer-Verlag Berlin, pp. 358–374.Google Scholar
  14. Kao, K.N. & M.R. Michayluk, 1975. Nutritional requirements for growth ofVicia hajastana cells and protoplasts at a very low population density in liquid media.Planta 126: 105–110.CrossRefGoogle Scholar
  15. Karp, A., 1990. Somaclonal variation in potato. In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry, Vol. 11, Springer-Verlag, Berlin, pp. 379–399.Google Scholar
  16. Magnien, E., 1981. Sensibilité des protoplastes deNicotiana sp. aux rayonnements ionisants. PhD Thesis, Université de Paris-Sud.Google Scholar
  17. Masson, J., M. Lecerf, P. Rousselle, P. Perennec & G. Pelletier, 1987. Plant regeneration from protoplasts of diploid potato derived from crosses ofSolanum tuberosum with wildSolanum species.Plant Science 53: 167–176.CrossRefGoogle Scholar
  18. Miller, R.A., O.L. Gamborg, W.A. Keller & K.N. Kao, 1971. Fusion and division of nuclei in multinucleated soybean protoplasts.Canadian Journal of Genetics and Cytology 13: 347–353.Google Scholar
  19. Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures.Physiological Plantarum 15: 473–497.Google Scholar
  20. Nagata, T. & I. Takebe, 1970. Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts.Planta 92: 301–308.CrossRefGoogle Scholar
  21. Pijnacker, L.P. & K. Sree Ramulu, 1990. Somaclonal variation in potato: a karyotypic evaluation.Acta Botanica Neerlandica 39 (2): 163–169.Google Scholar
  22. Pijnacker, L.P., K. Sree Ramulu, P. Dijkhuis & M.A. Ferwerda, 1989. Flow cytometric and karyological analysis of polysomaty and polyploidization during callus formation from leaf segments of various potato genotypes.Theoretical and Applied Genetics 77: 102–110.CrossRefGoogle Scholar
  23. Sree Ramulu, K., 1987. Case histories of genetic variability in vitro: potato. In: I.K. Vasil (Ed.), Cell Culture and Somatic Cell Genetics of Plants, Vol. 3, Academic Press, London New York, pp. 449–473.Google Scholar
  24. Sree Ramulu, K., 1987. Genetic instability during plant regeneration in potato: origin and implications.Plant Physiology (Life Sci. Adv.) 6: 211–218.Google Scholar
  25. Sree Ramulu, K., P. Dijkhuis & S. Roest, 1983. Phenotypic variation and ploidy level of plants regenerated from protoplasts of tetraploid potato (Solanum tuberosum L. cv. Bintje).Theoretical and Applied Genetics 65: 329–338.CrossRefGoogle Scholar
  26. Sree Ramulu, K., P. Dijkhuis & S. Roest, 1989. Patterns of phenotypic and chromosome variation in plants derived from protoplast cultures of monohaploid, dihaploid and diploid genotypes and in somatic hybrids of potato.Plant Science 60: 101–110.Google Scholar
  27. Sree Ramulu, K., P. Dijkhuis, S. Roest, G.S. Bokelmann & B. de Groot, 1984. Early occurrence of genetic instability in protoplast cultures of potato.Plant Science Letters 36: 79–86.CrossRefGoogle Scholar
  28. Waara, S., A. Wallin, A. Ottosson & T. Eriksson, 1991. Factors promoting sustained divisions of mesophyll protoplasts isolated from dihaploid clones of potato (Solanum tuberosum L.) and a cytological analysis of regenerated plants.Plant Cell, Tissue and Organ Culture 27: 257–265.CrossRefGoogle Scholar
  29. Widholm, J.M., 1972. The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells.Stain Technology 47: 189–194.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • W. J. Van Everdink
    • 1
  • L. P. Pijnacker
    • 1
  1. 1.Department of Genetics, Biological CentreUniversity of GroningenHarenThe Netherlands

Personalised recommendations