Skip to main content
SpringerLink
Log in

Effect of inorganic nitrogen nutrition on cytokinin-induced potato microtuber production in vitro

  • Full Papers
  • Published:
Potato Research Aims and scope Submit manuscript

Summary

The effect of inorganic nitrogen nutrition on the induction and development of microtubers by cytokinin-induced tuberization was studied in four potato genotypes belonging to different maturity groups. The objective of this study was to investigate whether a reduction in total nitrogen level in the Murashige & Skoog medium would improve cytokinin-induced tuberization rate. The effect of three levels of total nitrogen (15, 30 and 45 meq) on tuberization was studied at constant (20 meq K) and varying potassium levels approximating to 5, 10 and 15 meq. Reducing the total nitrogen supply increased the number but decreased the size of nitrogen level on the rate of assimilate partitioning (harvest index) during cytokinin-induced microtuberization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Chandra, R., G.J. Randhawa, D.R. Chaudhari & M.D. Upadhaya, 1992. Efficacy of triazole for in vitro microtuber production in potato.Potato Research 35: 339–341.

    CAS  Google Scholar 

  • Estrada, R., P. Tovar & J.H. Dodds, 1986. Induction of in vitro tubers in a broad range of potato genotypes.Plant Cell, Tissue and Organ Culture 7: 3–10.

    Article  Google Scholar 

  • Garner, N. & J. Blake, 1989. The induction and development of potato microtubers in vitro on media free of growth regulating substances.Annals of Botany 63: 663–674.

    CAS  Google Scholar 

  • Gomez, K.A. & A.A. Gomez, 1984. Statistical Procedures for Agricultural Research. John Wiley & Sons, New York.

    Google Scholar 

  • Jones, E.D., 1988. A current assessment of in vitro culture and other rapid multiplication methods in North America and Europe.American Potato Journal 65: 209–220.

    Google Scholar 

  • Krauss, A., 1985. Interaction of nitrogen nutrition, phytohormones, and tuberization. In: P.H. Li (Ed.), Potato Physiology. Academic Press, London, pp. 209–230.

    Google Scholar 

  • Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures.Physiologia Plantarum 15: 473–497.

    CAS  Google Scholar 

  • Naik, P.S. & D. Sarkar, 1997. Influence of light-induced greening on storage of potato microtubers.Biologia Plantarum 39: 31–34.

    Article  Google Scholar 

  • Okazawa, Y., 1967. Physiological studies on the tuberization of potato plants.Journal Faculty of Agriculture Hokkaido University 55: 267–336.

    Google Scholar 

  • Palmer, C.E. & O.E. Smith, 1969. Cytokinins and tuber initiation in the potatoSolanum tuberosum L.Nature 221: 279–280.

    CAS  Google Scholar 

  • Pelacho, A.M. & A.M. Mingo-Castel, 1991. Jasmonic acid induces tuberization of potato stolons cultured in vitro.Plant Physiology 97: 1253–1255.

    CAS  Google Scholar 

  • Sarkar, D., R. Chandra & P.S. Naik, 1997. Effect of inoculation density on potato micropropagation.Plant Cell, Tissue and Organ Culture 48: 63–66.

    Article  CAS  Google Scholar 

  • Sattelmacher, B. & H. Marschner, 1978a. Nitrogen nutrition and cytokinin activity inSolanum tuberosum.Physiologia Plantarum 42: 185–189.

    CAS  Google Scholar 

  • Sattelmacher, B. & H. Marschner, 1978b. Relation between nitrogen nutrition, cytokinin activity and tuberization inSolanum tuberosum.Physiologia Plantarum 44: 65–68.

    CAS  Google Scholar 

  • Stallknecht, G.F. & S. Farnsworth, 1979. The effect of nitrogen on the coumarin-induced tuberization of potato axillary shoots cultured in vitro.American Potato Journal 56: 523–530.

    CAS  Google Scholar 

  • Tovar, P., R. Estrada, L. Schilde-Rentschler & J.H. Dodds, 1985. Induction of in vitro potato tubers.CIP Circular 13: 1–4.

    Google Scholar 

  • Wang, P.-J. & C.-Y. Hu, 1982. In vitro mass tuberization and virus-free seed-potato production in Taiwan.American Potato Journal 59: 33–37.

    Google Scholar 

  • Wang, P.-J. & C.-Y. Hu, 1985. Potato tissue culture and its application in agriculture. In: P.H. Li (Ed.), Potato Physiology. Academic Press, London, pp. 503–577.

    Google Scholar 

  • Wattimena, G.A., 1983. Micropropagation as an alternative technology for potato production in Indonesia. PhD Thesis, University of Wisconsin, Madison.

    Google Scholar 

  • Zarrabeitia, A., X. Lejarcegui, J. Veramendi & A.M. Mingo-Castel, 1997. Influence of nitrogen supply on micropropagation and subsequent microtuberization of four potato cultivars.American Potato Journal 74: 369–378.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Genetics & Plant Breeding, Central Potato Research Institute, Shimla-171 001, Himachal Pradesh, India

    Debabrata Sarkar & Prakash S. Naik

Authors
  1. Debabrata Sarkar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Prakash S. Naik
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sarkar, D., Naik, P.S. Effect of inorganic nitrogen nutrition on cytokinin-induced potato microtuber production in vitro. Potato Res 41, 211–217 (1998). https://doi.org/10.1007/BF02358191

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02358191

Additional keywords

Search

Navigation