In vitro induction of potato tuberization by organic acids
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Potato microtubers are usually induced in media containing growth regulatory, typically cytokinins and growth retardants. However, since these substances may imbalance the physiology of the cultured explants and cause adverse carry-over effects on the subsequent performance of the microtubers, the potential acid-induction of in vitro-mass tuberization was investigated as an alternative. For this purpose, a range of explant types (stolons, single-node or apex-containing multinodal sections), organic acids (acetic, propionic, ascorbic, acetylsalicylic or salicylic acid), and photoperiods (continuous dark, 8 or 16 h photoperiod) were established. Gellified medium or raft membranes on liquid medium were also tested.
Although variations due to explant type, photoperiod, organic acid supplemented, and medium type were found, all organic acids tested under the different experiments caused tuberization. Multiple tuber formation or bigger tubers did not develop on multinodal explants. In terms of tuberization rate and mean tuber weight, gellified medium performed better than liquid medium with rafts. The recently established role of the salicylic and acetylsalicylic acids on tuberization is corroborated by our system. The results with the acetic, propionic and ascorbic acids show that in vitro hormone-free tuberization can be easily and rapidly achieved.
Additional keywordsSolanum tuberosum L. microtubers acetic acid propionic acid asscorbic acid salicylic acid acetylsalicylic acid
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- Dodds, J.H., D. Silva-Rodriguez & P. Tovar, 1992. Micropropagation of potato (Solanum tuberosum L.). In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry, vol. 19. High-Tech and Micropropagation III. Springer-Verlag, Berlin, Heildelberg, New York, pp. 91–106.Google Scholar
- Ewing, E.E., 1995. The role of hormones in potato (Solanum tuberosum L.) tuberization. In: P.J. Davies (Ed.), Plant hormones. Physiology, biochemistry and molecular biology, Kluwer, Dordrecht, pp. 698–724.Google Scholar
- Garner, N. & J. Blake, 1989. The induction and development of potato microtubersin vitro on media free of growth regulating substances.Annals of Botany 63:663–674.Google Scholar
- Gaspar, T., C. Kevers, C. Penel, H. Greppin, D.M. Reid & T.A. Thorpe, 1996. Plant hormones and plant growth regulators in plant tissue culture.In Vitro Cellular & Developmental Biology Plant 32:272–289.Google Scholar
- Hussey, G. & N.J. Stacey, 1984. Factors affecting the formation ofin vitro tubers of potato (Solanum tuberosum L.).Annals of Botany 53:565–578.Google Scholar
- Jones, E.D., 1988. A current assessment ofin vitro culture and other rapid multiplication methods in North-America and Europe.American Potato Journal 65:209–220.Google Scholar
- Leclerc, Y., D. Donnelly, W.K. Coleman & R.R. King, 1995. Microtuber dormancy in three potato cultivars.American Potato Journal 72:215–223.Google Scholar
- Levy, D., J.E.A. Seabrook & S. Coleman, 1993. Enhancement of tuberization of axillary shoot buds of potato (Solanum tuberosum L.) cultivars culturedin vitro.Journal of Experimental Botany 44(259):381–386.Google Scholar
- Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures.Physiologia Plantarum 15:473–497.Google Scholar
- Palmer, C.E. & O.E. Smith, 1970. Effect of kinetin on tuber formation on isolated stolons ofSolanum tuberosum L. culturedin vitro.Plant and Cell Physiology 11:303–314.Google Scholar
- Pelacho, A.M. & A.M. Mingo-Castel, 1991a. Effects of photoperiod on kinetin-induced tuberization of isolated potato stolons culturedin vitro.American Potato Journal 68:533–541.Google Scholar
- Pelacho, A.M. & A.M. Mingo-Castel, 1991b. Jasmonic acid induces tuberization of potato stolons culturedin vitro.Plant Physiology 97:1253–1255.Google Scholar
- Sanfeliu, J.Ll. & A.M. Pelacho, 1994.In vitro induction of stolon tuberization by acid substances inSolanum tuberosum L. Abstracts of the VIIIth International Congress on Plant Tissue and Cell Culture, Firenze, p. 219.Google Scholar
- Slimmon, T., V. Souza-Machado & R. Coffin, 1989. The effect of light onin vitro microtuberization of potato cuttings.American Potato Journal 66:843–848.Google Scholar
- Tagawa, T. & Y. Okazawa, 1952. Physiological and morphological studies on potato plants. Part 10. On the physiological behaviour of ascorbic acid in the formation of potato tubers.Memoirs of the Faculty of Agriculture Hokkaido University, Sapporo 1:240–245.Google Scholar
- Tovar, P., R. Estrada, L. Schilde-Rentschler & J.H. Dodds, 1985. Induction and use ofin vitro potato tubers.CIP circular 13:1–5.Google Scholar
- Vreugdenhil, D. & P.C. Struik, 1989. An integrated view of the hormonal regulation of tuber formation in potato (Solanum tuberosum).Physiologia Plantarum 75:521–531.Google Scholar
- Wan, W.Y., W. Cao & T.W. Tibbits, 1994. Tuber initiation in hydroponically grown potatoes by alteration of solution pH.HortScience 29:621–623.Google Scholar