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Rapid detection of potato viruses by dot-ELISA

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Summary

The dot-ELISA, an immunosorbent assay on nitrocellulose (NC) membranes, was applied to potato viruses. The sensitivity of the test, as determined by visual assessment of the colour reactions in serial dilutions, allowed a reliable detection of the potato viruses M, S, X, Y, A and of potato leafroll virus in leaves of non-potato hosts and potato plants. Tuber extracts taken from the rose end resulted in higher dilution end points than those from the heel end. The test can be performed within two to three hours by using stored NC-membranes precoated with specific γ-globulin. The possibility of storing antigen treated NC-membranes for some days allows the assay to be quickly applied to samples taken in the field.

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References

  • Banttari, E. E. & P. H. Goodwin, 1985. Detection of potato viruses, S, X, Y by enzyme-linked immunosorbent assay on nitrocellulose membranes (Dot-ELISA).Plant Disease 69: 202–205.

    Google Scholar 

  • Barker, H. & B. D. Harrison, 1985. Restricted multiplication of potato leafroll virus in resistant potato genotypes.Annals of Applied Biology 107: 205–212.

    Google Scholar 

  • Berger, P. H., D. W. Thornbury & T. P. Pirone, 1985. Detection of picogram quantities of potyviruses using a dot blot immunobinding assay.Journal of Virological Methods 12: 31–39.

    Article  CAS  PubMed  Google Scholar 

  • Bode, L., L. Beutin & H. Köhler, 1984. Nitrocellulose-enzyme-linked immunosorbent assay (NC-ELISA) — a sensitive technique for the rapid visual detection of both viral antigens and antibodies.Journal of Virological Methods 8: 111–121.

    Article  CAS  PubMed  Google Scholar 

  • Clark, M. F. & A. N. Adams, 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses.Journal of General Virology 34: 475–485.

    CAS  PubMed  Google Scholar 

  • Graddon, D. J. & J. W. Randles, 1986. Single antibody dot immunoassay — a simple technique for rapid detection of a plant virus.Journal of Virological Methods 13: 63–69.

    Article  CAS  PubMed  Google Scholar 

  • Gugerli, P. & W. Gehriger, 1980. Enzyme-linked immunosorbent assay (ELISA) for the detection of potato leafroll virus and potato virus Y in potato tubers after artificial break of dormancy.Potato Research 23: 353–359.

    Article  Google Scholar 

  • Hawkes, R., E. Niday & J. Gordon, 1982. A dot-immunobinding assay for monoclonal and other antibodies.Analytical Biochemistry 119: 142–147.

    Article  CAS  PubMed  Google Scholar 

  • Hibi, T. & Y. Saito, 1985. A dot immunobinding assay for the detection of tobacco mosaic virus in infected tissues.Journal of General Virology 66: 1191–1194.

    Google Scholar 

  • Loh, P. C., M. A. Dow & R. S. Fujioka, 1985. Use of the nitrocellulose-enzyme immunosorbent assay for rapid, sensitive and quantitative detection of human enteroviruses.Journal of Virological Methods 12: 225–234.

    Article  CAS  PubMed  Google Scholar 

  • Powell, C. A., 1987. Detection of three plant viruses by dot-immunobinding assay.Phytopathology 77: 306–309.

    CAS  Google Scholar 

  • Singh, R. P. & T. H. Somerville, 1987. Relationship of virus concentration with the field resistance to potato virus Y in potatoes.American Potato Journal 64: 69–80.

    Google Scholar 

  • Smith, F. D. & E. E. Banttari, 1987. Dot-ELISA on nitrocellulose membranes for detection of potato leafroll virus.Plant Disease 71: 795–799.

    Google Scholar 

  • Tamada, T. & B. D. Harrison, 1980. Application of enzyme-linked immunosorbent assay to the detection of potato leafroll virus in potato tubers.Annals of Applied Biology 96: 67–78.

    Google Scholar 

  • Towbin, H., T. Stachelin & J. Gordon, 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications.Proceedings of the National Academy of Science USA 76: 4350–4354.

    CAS  Google Scholar 

  • Vetten, H. J., U. Ehlers & H.-L. Paul, 1983. Detection of potato virus Y and A in tubers by enzyme-linked immunosorbent assay after natural and artificial break of dormancy.Phytopathologische Zeitschrift 108: 41–53.

    Google Scholar 

  • Weidemann, H.-L., 1984. Die Kartoffelvirus Y (PVYN)-Konzentration in Knollen verschiedener Kartoffelsorten.Nachtrichtenblatt des Deutschen Pflanzenschutzdienstes (Braunschweig) 36: 25–27.

    Google Scholar 

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  1. Institut für Viruskrankheiten der Pflanzen, Biologische Bundesanstalt für Land- und Forstwirtschaft, Messeweg 11/12, D-3300, Braunschweig, Federal Republic of Germany

    Hans-L. Weidemann

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  1. Hans-L. Weidemann
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Weidemann, HL. Rapid detection of potato viruses by dot-ELISA. Potato Res 31, 485–492 (1988). https://doi.org/10.1007/BF02357886

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  • DOI: https://doi.org/10.1007/BF02357886

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