Advantages of a soluble dye in blot immunobinding assays for virus detection in potatoes
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A technique is described in which plant sap is blotted onto small pieces (8×11 mm) of nylon membrane and virus particles bound to the paper are detected by a modification of the enzymelinked immunosorbent assay (ELISA). The detectable product of the assay is a soluble yellow dye, the absorbance of which increased with the virus content of the plant sap. Leaf or tuber sap from plants secondarily-infected with either potato leafroll virus or potato virus Y could be clearly distinguished from that of healthy plants and a majority of tubers primarily infected with PVY were also detected.
Additional keywordspotato leafroll virus potato virus Y virus diagnosis
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