Summary
Potato leafroll virus and potato viruses M, S, X and Y in green leaves were detected by dot immunobinding (DIB) on plain paper and on nitrocellulose membranes. On both materials, DIB could detect the presence of very small amounts of virus, e.g. 30 pg of purified PVX. The sensitivity of the DIB test on plain paper and on nitrocellulose was compared to Double Antibody Sandwich ELISA (DAS-ELISA) by serial dilutions of infected plant sap made in healthy plant sap. Detection of potato viruses by DIB on plain paper and nitrocellulose was found to be equally sensitive whereas DAS-ELISA was 2 to 8 times more sensitive. Possible simplifications of the DIB procedure to suit the requirements of a routine method were examined. The use of the DIB method for routine testing of potato viruses is discussed.
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References
Avrameas, S. & T. Ternynck, 1971. Peroxidase-labelled antibody and fab conjugates with enhanced intracellular penetration.Immunochemistry 8: 1175–1179.
Banttari, E. E. & P. H. Goodwin, 1985. Detection of potato viruses S, X, and Y by enzyme-linked immunosorbent assay on nitrocellulose membranes (Dot-ELISA).Plant Disease 69: 202–205.
Blake, M. S., K. H. Johnson, G. J. Russel-Jones & E. C. Gotschlich, 1984. A rapid, sensitive method for detection of alkaline phosphate-conjugated anti-antibody on western blots.Analytical Biochemistry 136: 175–179.
Clark, M. F. & A. N. Adams, 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses.Journal of General Virology 34: 475–483.
Lange, L. & M. Heide, 1986. Dot immuno binding (DIB) for detection of virus in seed.Canadian Journal of Plant Pathology 8: 373–379.
Lange, L. & M. Heide, 1987. Testing of seeds for viruses by dot immunoblotting (DIB) directly on plain paper. (Submitted for publication).
Leach, J. E., B. A. Ramundo, D. L. Pearson & L. E. Claflin, 1987. Dot-immunobinding assay for detectingXanthomonas campestris pv.holicola in sorghum.Plant Disease 71: 30–33.
Lockhart, B. E. L., M. el Maataoui, T. W. Carroll, A. M. Lennon & S. K. Zaske, 1986. Identification of barley yellow striate mosaic virus in Morocco and its field detection by enzyme immune assay.Plant Disease 70: 1113–1117.
Malin, E., E. L. Belden & D. Roth, 1985. Evaluation of the radioimmunoassay, indirect enzymelinked immunosorbent assay, and dot blot assay for the identification ofXanthomonas campestris pv.phaseoli.Canadian Journal of Plant Pathology 7: 217–340.
Powell, C. A., 1987. Detection of three plant viruses by dot-immunobinding assay.Journal of Phytopathology 77: 306–309.
Reichmann, M. E., 1960. Degradation of potato virus X.Journal of Biological Chemistry 235: 2959–2963.
Sherwood, J. L., 1987. Comparison of a filter paper immunobinding assay, western blotting and an enzyme-linked immunosorbent assay for the detection of wheat streak mosaic virus.Journal of Phytopathology 118: 68–75.
Vissing, H. & O. D. Madsen, 1984. Comparison of detection limits for various nitrocellulose binding immunoassays using Beta-2 microglobulin as a model antigen.Electrophoresis 5: 313–314.
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Heide, M., Lange, L. Detection of potato leaf roll virus and potato viruses M, S, X and Y by dot immunobinding on plain paper. Potato Res 31, 367–373 (1988). https://doi.org/10.1007/BF02357870
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DOI: https://doi.org/10.1007/BF02357870