A microplate reader assay for rapid enzymatic quantification of sugars in potato tubers
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A sensitive, rapid and inexpensive method has been developed for quantifying the glucose, fructose and sucrose content of potato tubers. The method, based on selective enzyme-coupled reaction systems and the reduction of NAD to NADH, uses a microplate reader fitted with a 340 nm filter. In one microtiter plate 96 samples can be analysed for all three sugars in less than two hours. Several plates can be processed in parallel.
Additional keywordsglucose fructose sucrose Solanum tuberosum L.
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