Advertisement

Potato Research

, Volume 35, Issue 1, pp 55–58 | Cite as

A microplate reader assay for rapid enzymatic quantification of sugars in potato tubers

  • R. Viola
  • H. V. Davies
Short Communication

Summary

A sensitive, rapid and inexpensive method has been developed for quantifying the glucose, fructose and sucrose content of potato tubers. The method, based on selective enzyme-coupled reaction systems and the reduction of NAD to NADH, uses a microplate reader fitted with a 340 nm filter. In one microtiter plate 96 samples can be analysed for all three sugars in less than two hours. Several plates can be processed in parallel.

Additional keywords

glucose fructose sucrose Solanum tuberosum L. 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baijal, B. D. & W. F. van Vliet, 1966. The chemical composition in different parts of the potato tuber during storage.European Potato Journal 9: 179–190.Google Scholar
  2. Cerning-Beroard, M., 1975. A note on sugar determination by the anthrone method.Cereal Chemistry 52: 875–860.Google Scholar
  3. Cronin, D. A. & S. Smith, 1979. A simple and rapid procedure for the analysis of reducing, total and individual sugars in potatoes.Potato Research 22: 99–105.Google Scholar
  4. Davies, H. V., 1988. Rapid determination of glucose, fructose and sucrose in potato tubers by capillary gas chromatography.Potato Research 31: 569–572.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • R. Viola
    • 1
  • H. V. Davies
    • 1
  1. 1.Department of Cellular and Environmental PhysiologyScottish Crop Research InstituteDundeeUK

Personalised recommendations