Summary
Application of two lignin chemistry techniques, CuO oxidation and thioglycolic acid derivatization, has confirmed that natural and wound-induced suberin does have a lignin-like component. The time course of suberin phenolic deposition could be monitored by using the thioglycolic acid technique. The thioglycolic acid procedure is easily performed, can be adapted readily to multiple samples, and thus could be an effective tool for studying rates of wound-induced suberization.
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Hammerschmidt, R. Determination of natural and wound-induced potato tuber suberin phenolics by thioglycolic acid derivatization and cupric oxide oxidation. Potato Res 28, 123–127 (1985). https://doi.org/10.1007/BF02357578
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DOI: https://doi.org/10.1007/BF02357578