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Methods for assessing bacterial density on potato tubers

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Summary

Formulae which determined tuber surface area from tuber dimensions and weight were tested by using samples from three potato cultivars. Surviving populations of a labelled isolate ofErwinia carotovora ssp.atroseptica following planting, and populations of a fluorescent pseudomonad following dusting with a bacterial powder formulation were used to demonstrate the application of these formulae to the calculation of bacterial density on the tuber surface. Results obtained by washing whole tubers using the formulae to calculate the surface area were shown to be comparable in sensitivity with those obtained by dilution-plating of periderm discs of 1cm2.

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References

  • Burton, W. G., 1966. The Potato. Veenman, Wageningen.

    Google Scholar 

  • Geels, F. P. & B. Schippers, 1983. Selection of antagonistic fluorescentPseudomonas spp. and their root colonization and persistence following treatment of seed potatoes.Phytopathologische Zeitschrift 108: 193–206.

    Google Scholar 

  • Harris, P. M., 1978. The Potato Crop. Chapman & Hall, London.

    Google Scholar 

  • Howie, W. J. & E. Echandi, 1983. Rhizobacteria: influence of cultivar and soil type on plant growth and yield of potato.Soil Biology and Biochemistry 15: 127–132.

    Article  Google Scholar 

  • King, E. O., M. K. Ward & D. E. Raney, 1954. Two simple media for the demonstration of pyocyanin and fluorescin.Journal of Laboratory and Clinical Medicine 44: 301–307.

    CAS  PubMed  Google Scholar 

  • Kloepper, J. W., 1983. Effect of seed piece inoculation with plant growth-promoting rhizobacteria on populations ofErwinia carotovora on potato roots and daughter tubers.Phytopathology 73: 217–219.

    Google Scholar 

  • Kloepper, J. W. & M. N. Schroth, 1981. Development of a powder formulation of rhizobacteria for inoculation of potato seed pieces.Phytopathology 71: 590–592.

    Google Scholar 

  • Logan, C., 1963. A selective medium for the isolation of soft rot coliforms from soil.Nature 199: 623.

    Google Scholar 

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Rhodes, D.J., Logan, C. Methods for assessing bacterial density on potato tubers. Potato Res 27, 419–422 (1984). https://doi.org/10.1007/BF02357429

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  • DOI: https://doi.org/10.1007/BF02357429

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