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Preproceruloplasmin is a primary product of cell-free translation of ceruloplasmin messenger RNA

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Summary

Biosynthesis of ceruloplasmin was studied in wheat germ extract programmed with polysomal RNA from rat liver. Optimal potassium concentration for the total protein-synthesizing activity and for the synthesis of immunoreactive ceruloplasmin was 96 and 186 mM respectively. 7-methylguanosine 5′-monophosphate caused two-fold inhibition of the cell-free synthesis of ceruloplasmin. Immunoprecipitated ceruloplasmin that was synthesized at optimal potassium concentration was a homogeneous polypeptide of a molecular weight about 84 kD. The addition of membrane fractions from rat liver to the incubation mixture caused the conversion of the 84 kD polypeptide into 80 kD and 65 kD polypeptides that are similar to proceruloplasmins synthesized in rat liver during in vivo pulse labelling. The suggestion is made that 84 kD polypeptide is a primary product of the translation of ceruloplasmin mRNA (preproceruloplasmin).

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Abbreviations

CP:

ceruloplasmin

SDS:

sodium dodecyl sulfate

TCA:

trichloracetic acid

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Authors and Affiliations

  1. Institute for Experimental Medicine of the USSR Academy of Medical Sciences, 197022, Leningrad, USSR

    L. V. Puchkova, V. S. Gaitskhoki, N. K. Monakhov, L. T. Timchenko & S. A. Neifakh

Authors
  1. L. V. Puchkova
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  2. V. S. Gaitskhoki
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  3. N. K. Monakhov
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  4. L. T. Timchenko
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  5. S. A. Neifakh
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Puchkova, L.V., Gaitskhoki, V.S., Monakhov, N.K. et al. Preproceruloplasmin is a primary product of cell-free translation of ceruloplasmin messenger RNA. Mol Cell Biochem 35, 159–169 (1981). https://doi.org/10.1007/BF02357086

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  • DOI: https://doi.org/10.1007/BF02357086

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