Summary
The oxygen equilibrium of human hemoglobin has been studied in the presence of 1-benzyl-3-indazoleoxyacetate (BZ).
The results show that:
-
(a)
The overall oxygen affinity of hemoglobin is a function of BZ concentration, but the cooperative character of the equilibrium curve appears insensitive to the drug up to the maximal concentration studied (5 × 10−2 M);
-
(b)
The functional expression of the interaction between hemoglobin and BZ is not affected by the presence of protons, i.e., the change in oxygen affinity determined by BZ is the same at any pH value studied;
-
(c)
The aromatic region of BZ molecules is of primary importance for the functional change of hemoglobin;
-
(d)
The difference in moles of BZ bound per mole of tetrameric unliganded and oxygenated hemoglobin corresponds to 2; these functionally relevant binding sites on the protein are probably located at α1, β1 and α2β2 interfaces;
-
(e)
Thermodynamically, entropy effects dominate the reaction between hemoglobin and BZ.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Hichens, M., 1974. Antinflammatory Agents, Chemistry and Pharmacology, (Scherrer R. A. & Whitehouse M. W., Eds.), vol. II, pp 263–302. Academic Press, New York.
Mizushima, Y., 1954. Ahchs. Int. Pharmacodyn. Ther. 149: 1–8.
Zolla, L., Amiconi, G. & Catanese, B., 1977. Biochem. Pharmacol. 26: 659–662.
Kiehs, K., Hansch, C. & Moore, L., 1966. Biochemistry 5: 2602–2605.
Rossi Fanelli, A., Antonini, E. & Caputo, A., 1961. J. Biol. Chem. 236: 397–400.
Rossi Fanelli, A. & Antonini, E., 1958. Arch. Biochem. 77: 478–492.
Hill, A. W., 1910. J. Physiol. 40: 4–7.
Edsall, J. T. & Wyman, J., 1958. Biophysical Chemistry, vol. I, ch. 11. Academic Press, New York.
Wyman, J., 1964. Advances in Protein Chemistry 19: 223–286. Academic Press, New York.
Wyman, J., 1967. J. Am. Chem. Soc. 89: 2202–2218.
Antonini, E. & Brunori, M., 1971. Hemoglobin and Myoglobin in their Reaction with Ligands, North-Holland, Amsterdam.
Bates, G., Brunori, M., Amiconi, G., Antonini, E. and Wyman, J., 1968. Biochemistry, 7: 3016–3020.
Antonini, E., Wyman, J., Moretti, R. and Rossi Fanelli, A., 1963. Biochim. Biophys. Acta, 71: 124–138.
Antonini, E., Wyman, J., Brunori, M. and Rossi Fanelli, A., 1965. Biochim. Biophys. Acta, 94: 188–193.
Steinhardt, J. and Reynolds, J. A., 1969. Multiple Equilibria in Proteins, Academic Press, New York.
Wishnia, A., 1969. Biochemistry, 8: 5064–5070.
Wishnia, A., 1969. Biochemistry, 8: 5070–5075.
Perutz, M. F., Muirheard, H., Cox, J. M. and Goamon, L. C. G., 1968, Nature 219: 131–139.
Silvestrini, B., Catanese, B. and Ciolo, V. (June 1973), International Meeting on Future Trends in Inflammation, pp. 28–30, Verona (Italy).
Laver, M. B., 1975. Oxygen Measurements in Biology and Medicine (Payne, J. P. & Hill, D. W., eds.) pag. 5, Butterworth, London.
Gross, G. J., Waltier, D. C. and Hardman, H. F., 1977. J. Pharm. Exp. Therap. 203: 72–76.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Amiconi, G., Zolla, L. & Catanese, B. Binding of organic ions by proteins. Mol Cell Biochem 36, 143–146 (1981). https://doi.org/10.1007/BF02357030
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02357030