Molecular and Cellular Biochemistry

, Volume 36, Issue 3, pp 135–141 | Cite as

Liver chromatin fractions inMus andAkodon

The concept of constitutive heterochromatin
  • Angel Catala
  • Lidia Vidal-Rioja
  • Nestor O. Bianchi
Article

Summary

The liver chromatin fromMus musculus andAkodon molinae was separated in 8 fractions by differential centrifugation. Like fractions from both species showed approximately similar contents of DNA, equivalent ratios of histone to non-histone proteins, corresponding template activities and equal amounts of positive C-banded material. On the other hand, heavy chromatin fractions ofMus were highly enriched in satellite DNA whereas no satellite DNA was found inAkodon chromatin.

Heavy chromatin fractions isolated by differential sedimentation have been usually homologued with the constitutive heterochromatin. The properties of the constitutive chromatin are discussed and the validity of the foregoing concept is challenged. It is proposed to define the constitutive heterochromatin as those chromatin regions comprising highly repeated DNA sequences clustered in restricted areas of chromosomes and not transcribed (satellite DNA).

Keywords

Restricted Area Differential Centrifugation Similar Content Equivalent Ratio Chromatin Region 

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Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1981

Authors and Affiliations

  • Angel Catala
    • 1
  • Lidia Vidal-Rioja
    • 1
  • Nestor O. Bianchi
    • 1
  1. 1.Departmento de Citogenética MolecularIMBICELa PlataArgentina

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