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Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations

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Summary

Cytosolic adenylate cyclase activity from rat seminiferous tubules was purified by chromatography in DEAE-cellulose, hydroxylapatite and Bio-Gel A-0.5 m as well as by centrifugation in sucrose gradients. In all these purification steps, fractions with adenylate cyclase activity also contained binding activity for L-T3. Binding studies indicate the existence of two L-T3 receptor components associated to adenylate cyclase activity. The component exhibiting the highest hormone affinity has the lowest binding capacity.

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Authors and Affiliations

  1. Instituto de Investigaciones Bioquímicas ‘Fundación Campomar’, Fac. de Ciencias Exactas y Naturales, Universidad Nacional de Tucumán, Obligado 2490, 1428, Buenos Aires, Argentina

    A. R. Kornblihtt (Fellow of the Consejo Nacional de Investigaciones Científicas y Técnicas and of the Comisión de Investigaciones Científicas (Provincia de Buenos Aires), M. M. Flawiá (Career investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina), D. de Mendoza (Career investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina), G. Glikin (Fellow of the Consejo Nacional de Investigaciones Científicas y Técnicas and of the Comisión de Investigaciones Científicas (Provincia de Buenos Aires), R. Farías (Career investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina) & H. N. Torres (Career investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina)

  2. Instituto de Química Biológica, Universidad Nacional de Tucumán, Obligado 2490, 1428, Buenos Aires, Argentina

    A. R. Kornblihtt (Fellow of the Consejo Nacional de Investigaciones Científicas y Técnicas and of the Comisión de Investigaciones Científicas (Provincia de Buenos Aires), M. M. Flawiá (Career investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina), D. de Mendoza (Career investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina), G. Glikin (Fellow of the Consejo Nacional de Investigaciones Científicas y Técnicas and of the Comisión de Investigaciones Científicas (Provincia de Buenos Aires), R. Farías (Career investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina) & H. N. Torres (Career investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina)

Authors
  1. A. R. Kornblihtt
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  2. M. M. Flawiá
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  3. D. de Mendoza
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  4. G. Glikin
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  5. R. Farías
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  6. H. N. Torres
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Kornblihtt, A.R., Flawiá, M.M., de Mendoza, D. et al. Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations. Mol Cell Biochem 36, 23–27 (1981). https://doi.org/10.1007/BF02354828

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  • DOI: https://doi.org/10.1007/BF02354828

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