Skip to main content
SpringerLink
Log in

Porcineβ-lactoglobulin A and C

Occurrence, isolation and chemical properties

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

The occurrence of the dominant ‘whey’ protein in samples of milk from 1180 sows is examined. It exhibits genetic polymorphism with some unusual features. Although immunologically different from bovine β-lactoglobulin, it is shown by chemical studies of the isolated protein to be a β-lactoglobulin. Two homozygous genetic variants, designated porcine β-lactoglobulin A and C, are isolated and their amino acid compositions and peptide maps compared. It is shown that the C variant has +1 His, −1 Gln, and +1 Asp, −1 Glu, with respect to the A variant. These variants, containingca. 162 residues per molecule, are considered in relationship to porcine β-lactoglobulins isolated by other workers. The sequence of the first 50 residues is determined and compared with sequence of the bovine protein. The sequences ofca. 70% of the remaining residues is proposed on the basis of the composition of tryptic peptides and assumed homology.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bell, K. & McKenzie, H. A., 1964. Nature 204: 1275–1279.

    CAS  PubMed  Google Scholar 

  2. McKenzie, H. A., 1967. Advan. Protein Chem. 22: 55–234.

    CAS  Google Scholar 

  3. Nordbring, F., 1957. Acta Soc. Med. Uppsal. 62: 135–151.

    CAS  Google Scholar 

  4. Karlsson, B. W., 1965. Ark. Zool. 18: 33–50.

    Google Scholar 

  5. Karlsson, B. W., 1966. Acta Pathol. Microbiol. Scand 67: 83–101.

    CAS  PubMed  Google Scholar 

  6. Karlsson, B. W., 1966. Thesis, Institute of Zoophysiology, University of Lund, Sweden.

  7. Gordon, W. G., Semmett, W. F. & Ziegler, J., 1954. J. Amer. Chem. Soc. 76: 287.

    CAS  Google Scholar 

  8. Kraeling, R. R. & Gerrits, R. J., 1969. J. Dairy Sci. 52: 2036–2038.

    CAS  Google Scholar 

  9. Kalan, E. B., Kraeling, R. R. & Gerrits, R. J., 1968. Fed. Proc. 27: 773.

    Google Scholar 

  10. Kalan, E. B., Kraeling, R. R. & Gerrits, R. J., 1971. Int. J. Biochem. 2: 232–244.

    Article  CAS  Google Scholar 

  11. Kessler, E. & Brew, K., 1970. Biochim. Biophys. Acta 200: 449–458.

    CAS  PubMed  Google Scholar 

  12. Bell, K., McKenzie, H. A. & Ralston, G. B., 1970. Proc. Aust. Biochem. Soc. 3: 82.

    Google Scholar 

  13. McKenzie, H. A., 1971. In: Milk Proteins (H. A. McKenzie, ed.), Vol. II, pp. 483–508, Academic Press, New York.

    Google Scholar 

  14. Armstrong, J. McD., McKenzie, H. A. & Sawyer, W. H., 1967. Biochim. Biophys. Acta 147: 60–72.

    CAS  PubMed  Google Scholar 

  15. Armstrong, J. McD., Hopper, K. E., McKenzie, H. A. & Murphy, W. H., 1970. Biochim. Biophys. Acta 214: 419–426.

    CAS  PubMed  Google Scholar 

  16. Bell, K., McKenzie, H. A., Muller, V., Rogers, C. & Shaw, D. C., 1980. Comp. Biochem. Physiol., in the press.

  17. Vandekerckhove, J. & van Montagu, M., 1974. Eur. J. Biochem. 44: 279–288.

    Article  CAS  PubMed  Google Scholar 

  18. Mendez, E., & Lai, C. Y., 1975. Analyt. Biochem. 68: 47–53.

    CAS  PubMed  Google Scholar 

  19. Bell, K., McKenzie, H. A. & Shaw, D. C., 1981. Molec. Cell. Biochem. 35, 113–119.

    CAS  PubMed  Google Scholar 

  20. Cooper, D. W., 1966. Anim. Prod. 8: 511–513.

    Google Scholar 

  21. Braunitzer, G., Chen, R., Schrank, B. & Stangl, A., 1972. Hoppe-Seyler's Z. Physiol. Chem. 353: 832–834.

    CAS  PubMed  Google Scholar 

  22. Lyster, R. L. J., Jenness, R., Phillips, N. I. & Sloan, R. E., 1966. Comp. Biochem. Physiol. 17: 967–971.

    CAS  PubMed  Google Scholar 

  23. Bell, K., Frier, R., McKenzie, H. A. & Ralston, G. B., 1981. Molec. Cell. Biochem. in publication.

Download references

Author information

Authors and Affiliations

  1. Dept. of Physiology, University of Queensland, St. Lucia, Australia

    K. Bell

  2. Dept. of Physical Biochemistry, John Curtin School of Medical Research, Australian National University, Canberra, A.C.T., Australia

    H. A. McKenzie & D. C. Shaw

Authors
  1. K. Bell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. A. McKenzie
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. C. Shaw
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bell, K., McKenzie, H.A. & Shaw, D.C. Porcineβ-lactoglobulin A and C. Mol Cell Biochem 35, 103–111 (1981). https://doi.org/10.1007/BF02354824

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02354824

Keywords

Search

Navigation