Bioscience Reports

, Volume 12, Issue 2, pp 101–108 | Cite as

Insulin-dependent release of 5′-nucleotidase and alkaline phosphatase from liver plasma membranes

  • Sandra Incerpi
  • Patrizia Baldini
  • Mario Lo Bello
  • Paolo Luly


Insulin treatment of isolated liver plasma membranes induced the release of 5′-nucleotidase and alkaline phosphatase. This effect was maximal at physiological hormone concentrations, being 36% and 17% for 5′-nucleotidase and alkaline phosphatase respectively, and was fully mimicked by the phosphatidylinositol specific phospholipase C (PI-PLC), thus confirming the presence of a glycosyl-phosphatidylinositol anchoring-system for these exofacial enzymatic proteins. The complete inhibition of insulin dependent enzyme release by neomycin is strongly supportive of an involvement of membrane-located PI-PLC activity. In addition, the insulin-like effect on enzyme release induced by the GTP non-hydrolysable analog, GTP-γ-S, and its sensitivity to the pertussis toxin are in favour of a mediatory role exerted by the G proteins system, in the transduction of some actions of insulin.

Key Words

insulin phospholipase C 5′-nucleotidase alkaline phosphatase glycosylphosphatidylinositol pertussis toxin plasma membrane, (rat liver) 



Phosphatidylinositol-specific phospholipase C


Guanosin-5′-O-(3-thiotriphosphate) tetralithium salt




pertussis toxin




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Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Sandra Incerpi
    • 1
  • Patrizia Baldini
    • 1
  • Mario Lo Bello
  • Paolo Luly
  1. 1.Department of BiologyUniversity of Rome “Tor Vergata”RomeItaly

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