Skip to main content
SpringerLink
Log in

Methode zur quantitativen Bestimmung von Homovanillinsäure, Vanillinmandelsäure und Vanillinsäure im Urin mit dünnschichtchromatographischer Trennung

  • Originalien
  • Published:
Klinische Wochenschrift Aims and scope Submit manuscript

Zusammenfassung

Eine neue Methode zur quantitativen Bestimmung der Homovanillinsäure, Vanillinmandelsäure und Vanillinsäure im Urin mit dünnschichtchromatographischer Trennung der Metabolite wird beschrieben. Anwendungsmöglichkeiten der Technik werden diskutiert.

Summary

A new technique for the quantitative determination of homovanillic acid, vanillmandelic acid and vanillic acid in Urine by the thin-layer chromatographic separation of the metabolites has been described. Applications of this technique have been discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literatur

  1. Carlsson, A., andB. Waldeck: A fluorimetric method for the determination of dopamine. Acta physiol. scand.44, 293 (1958).

    CAS  PubMed  Google Scholar 

  2. Crawford, T. B. B., andW. Law: Method for the estimation of adrenaline and noradrenaline in urine. J. Pharm. Pharmacol. (Lond.)10, 179 (1958).

    CAS  PubMed  Google Scholar 

  3. Crawford, T. B. B., andA. S. Outschoorn: The quantitativen tive separation of adrenaline and noradrenaline in biological fluids and tissue extracts. Brit. J. Pharmacol.6, 8 (1951).

    CAS  PubMed  Google Scholar 

  4. Darken, M. A.: Applications of fluorescent brighteners in biological techniques. Science133, 1704 (1961).

    CAS  PubMed  Google Scholar 

  5. Duggan, D. E., R. L. Bowman, B. B. Brodie, andS. Undenfriend: A spectrophotofluorometric study of compounds of biological interest. Arch. Biochem.68 (1957).

  6. Drujan, B. D., T. L. Sourkes, D. S. Layne, andG. F. Murphy: The differential determination of catecholamines in urine. Canad. J. Biochem.37, 1153 (1959).

    CAS  PubMed  Google Scholar 

  7. Euler, U. S. v. andI. Floding: A fluorimetric micromethod for differentialestimation of adrenaline and noradrenaline. Acta physiol. scand.33, Suppl.118, 45 (1955).

    Google Scholar 

  8. Euler, U. S. v., andI. Floding: Fluorimetric estimation of adrenaline and noradrenaline in urine. Differential estimation of adrenaline. Acta physiol. scand.33, Suppl.118, 57 (1955).

    Google Scholar 

  9. Euler, U. S. v.: The development and applications of the trihydroxyindole method for catecholamines Symposion on catecholamines. Bethesda, Maryland, Oct. 16–18, 1958, S. 262.

  10. Gaddum, J. H., W. S. Peart, andM. Vogt: The estimation of adrenaline and allied substances in blood. J. Physiol. (Lond.)108, 467 (1949).

    CAS  Google Scholar 

  11. Häggendal, J.: Fluorimetric determination of 3-O-methylated derivatives of adrenaline and noradrenaline in tissues and body fluids. Acta physiol. scand.56, 258 (1962).

    PubMed  Google Scholar 

  12. Häggendal, J.: On the use of strong exchange resins for determinations of small amounts of catechol amines. Scand. J. clin. Lab. Invest.14, No 5 (1962).

    Google Scholar 

  13. Häggendal, J.: An improved method for fluorimetric determination of small amounts of adrenaline and noradrenaline in plasma and tissues. Acta physiol. scand.59, 242 (1963).

    Google Scholar 

  14. Harrison, W. H.: Detection of intermediate oxidation states of adrenaline and noradrenaline by fluorescence spectrometric analysis. Arch. Biochem.101, 116 (1963).

    CAS  PubMed  Google Scholar 

  15. Harrison, W. H.: Ascorbic acid-induced fluorescence of a noradrenaline oxidation product. Biochem. biophys. Acta (Amst.)70, 705 (1963).

    Google Scholar 

  16. Knauff, H. G., u.G. Verbeek: Fluorimetrische Bestimmung von Adrenalin und Noradrenalin im Harn. Ergebnisse bei Gesunden und bei Patienten mit Hypertonus. Klin. Wschr.40, 18 (1962).

    CAS  PubMed  Google Scholar 

  17. Lauber, K.: Eine vereinfachte Methode zur Bestimmung von Adrenalin und Noradrenalin im Harn. Z. klin. Chem.2, 76 (1964).

    CAS  Google Scholar 

  18. Lund, A.: Fluorimetric determination of adrenaline in blood. Acta pharmacol. (Kbh)5, 75 (1949).

    CAS  Google Scholar 

  19. Lund, A.: Fluorimetric determination of adrenaline in blood. The chemical constitution of adrenolutine (the flurescent oxidation product of adrenaline). Acta pharmacol. (Kbh)5, 121 (1949).

    Google Scholar 

  20. Lund, A.: Fluorimetric determination of adrenaline in blood. A new sensitive and specific method. Acta pharmacol. (Kbh.)5, 231 (1949).

    CAS  Google Scholar 

  21. Lund, A.: Simultaneous fluorimetric determination of adrenaline and noradrenaline in blood. Acta pharmacol. (Kbh.)6, 137 (1950).

    CAS  Google Scholar 

  22. Palmer, J. F.: The use of β-thiopropionic acid for the analysis of mixtures of adrenaline and noradrenaline in plasma by the fluorometric trihydroxyindole method. W. Indian med. J.13, 38 (1964).

    CAS  PubMed  Google Scholar 

  23. Smith, E. R. B., andH. Weil-Malherbe: Metanephrine and normetanephrine in human urine: Method and results. J. Lab. clin. Med.60, 212 (1962).

    CAS  PubMed  Google Scholar 

  24. Weil-Malherbe, H., andA. D. Bone: The chemical estimation of adrenaline-like substances. Biochem. J.51, 311 (1952).

    CAS  PubMed  Google Scholar 

  25. Weil-Malherbe, H., andA. D. Bone: Fluorimetric estimation of adrenaline and noradrenaline in plasma. Biochem. J.67, 65 (1957).

    CAS  PubMed  Google Scholar 

  26. Weil-Malherbe, H.: The fluorimetric estimation of catechol compounds by the ethylenediamine condensation method. Symposium on catecholamines. Bethesda, Maryland, Oct. 16–18 (1958), S. 278.

  27. Coward, R. F., P. Smith, andO. S, Wilson: Paper chromatography of phenolic derivatives of phenylethylamine and some other diazo-reactive bases in urine. Clin. chim. Acta9, 381 (1964).

    Article  CAS  PubMed  Google Scholar 

  28. Miyake, H., H. Yoshida, andR. Imaizumi: Determination methods for urinary 3-methoxy-4-hydroxymandelic acid and 3,4-dihydroxymandelic acid. Jap. J. Pharmacol.12, 79 (1962).

    CAS  Google Scholar 

  29. Pisano, J. J., J. R. Crout, andD. Abraham: Determination of 3-methoxy-4-hydroxymandelic acid in urine. Clin. chim. Acta7, 285 (1962).

    Article  CAS  PubMed  Google Scholar 

  30. Ruthven, C. R. J., andM. Sandler: The estimation of homovanillic acid in urine. Biochem. J.83, 30 (1962).

    Google Scholar 

  31. Sato, T., Y. Wada, andM. Maebashi: Simultaneous determination of urinary vanillylmandelic acid and 5-hydroxyindoleacetic acid. Tohoku J. exp. Med.80, 1 (1963).

    CAS  PubMed  Google Scholar 

  32. Sen, N. P., andP. L. McGeer: Gas chromatography of phenolic and catecholic amines as the trimethylsilyl ethers. Biochem. biophys. Res. Commun.13, No5 (1963).

  33. Sharman, D. F.: A fluorimetric method for the estimation of 4-hydroxy-3-methoxyphenylacetic acid (homovanillic acid) and its identification in brain tissue. Brit. J. Pharmacol.20, 204 (1963).

    CAS  PubMed  Google Scholar 

  34. Studnitz, W. v., andA. Hanson: Determination of 3-methoxy-4-hydroxymandelic acid in urine by high-voltage paper electrophoresis. Scand. J. clin. Lab. Invest.11, 101 (1959).

    Google Scholar 

  35. Studnitz, W. v.: Methodische und klinische Untersuchungen über die Ausscheidung der 3-methoxy-4-hydroxymandelsäure im Urin. Scand. J. clin. Lab. Invest.12, Suppl. 48 (1960)

    Google Scholar 

  36. Sunderman, W., P. D. Cleveland, N. C. Law, andF. W. Sunderman: A method for the determination of 3-methoxy-4-hydroxymandelic acid (vanilmandelic acid) for the diagnosis of pheochromocytoma. Amer. J. clin. Path.34, 4, 293 (1960).

    Google Scholar 

  37. Wada, Y.: Quantitative determination of 3,4-dihydroxymandelic acid (doma) in human urine. Tohoku J. exp. Med.79, 389 (1963).

    CAS  PubMed  Google Scholar 

  38. Weil-Malherbe, H.: Studies on the estimation of 3-methoxy-4-hydroxy-mandelic acid (VMA) in human urine. Analyt. Biochem.7, 485 (1964).

    CAS  PubMed  Google Scholar 

  39. Weise, V. K., R. K. McDonald, andE. H. Labrosse: Determination of urinary 3-methoxy-3-hydroxymandelic acid in man. Clin. chim. Acta6, 79 (1961).

    Article  CAS  PubMed  Google Scholar 

  40. Williams, C. M.: Gas chromatography of urinary aromatic acids. Analyt. Biochem.4, 423 (1962).

    CAS  PubMed  Google Scholar 

  41. Williams, C. M., andR. H. Leonard: Microanalytical determination of dihydroxy aromatic acids by gas chromatography. Analyt. Biochem.5, 362 (1963).

    CAS  PubMed  Google Scholar 

  42. Woiwod, A. J., andR. Knight: The determination of 3-methoxy-4-hydroxymandelic acid in urine. J. clin. Path.14, 502 (1961).

    CAS  PubMed  Google Scholar 

  43. Sankoff, I., andT. L. Sourkes: Determination by thin-layer chromatography of urinary homovanillic acid in normal and disease states. Canad. J. Biochem.41, 1381 (1963).

    CAS  PubMed  Google Scholar 

  44. Segura-Cardona, R., u.K. Soehring: Dünnschichtchromatographischer Nachweis kleinster Mengen von Katecholaminen und deren Derivaten. Med. exp.10, 251 (1964).

    CAS  PubMed  Google Scholar 

  45. Schmid, E., u.N. Henning: Über den Nachweis der 3-Methoxy-4-hydroxy-mandel-säure im Harn. Klin. Wschr.41, 566 (1963).

    Article  CAS  PubMed  Google Scholar 

  46. Boscott, R. J., andW. T. Cooke: Ascorbic Acid Requirements and Urinary Excretion of p-Hydroxyphenylacetic Acid in Steatorrhoea and Macrozytic Anaemia. Quart. J. Med., N. S.23, 307 (1954).

    CAS  Google Scholar 

  47. Dirscherl, W., u.A. Wirtzfeld: Vanillinsäure im menschlichen Urin. Ihre Isolierung, Bestimmung und Herkunft. Hoppe-Seylers Z. physiol. Chem.336, 81 (1964).

    CAS  PubMed  Google Scholar 

  48. Dirscherl, W., u.B. Brisse: Vanillinsäure als Endprodukt des Abbaues von Adrenalin und Noradrenalin. Acta endocr. (Kbh.)45, 641 (1964).

    CAS  PubMed  Google Scholar 

  49. Dirscherl, W., H. Thomas u.H. Schriefers: Vanillinsäure als Endprodukt des Abbaues von Adrenalin und Noradrenalin. Acta endocr. (Kbh.)39, 385 (1962).

    CAS  PubMed  Google Scholar 

  50. Dirscherl, W., u.H. Thomas: Vanillinsäure als Endprodukt des Stoffwechsels von Adrenalin und Noradrenalin. II. Bildung von Vanilloylglycin. Acta endocr. (Kbh.)44, 101 (1963).

    CAS  PubMed  Google Scholar 

  51. Thomas, H., u.W. Dirscherl: Zur quantitativen Bestimmung von Phenolsäuren mit diazotiertem p-Aminophenyl-β-diäthylaminoäthylsulfon. Arzneimittel-Forsch. (Drug Res.)12, 429 (1962).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Aus der Nervenklinik und der Medizinischen Klinik der Universität Erlangen-Nürnberg, Germany

    N. A. Tautz, G. Voltmer & E. Schmid

Authors
  1. N. A. Tautz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Voltmer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Schmid
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Meinem verehrten Lehrer, Herrn Prof. Dr. Dr.Wilhelm Dirscherl, zum 65. Geburtstag in Dankbarkeit gewidmet.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tautz, N.A., Voltmer, G. & Schmid, E. Methode zur quantitativen Bestimmung von Homovanillinsäure, Vanillinmandelsäure und Vanillinsäure im Urin mit dünnschichtchromatographischer Trennung. Klin Wochenschr 43, 233–235 (1965). https://doi.org/10.1007/BF02350864

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02350864

Search

Navigation