Antonie van Leeuwenhoek

, Volume 47, Issue 4, pp 297–306 | Cite as

Effect of NaCl on kinetics ofd-glucosamine uptake in yeasts differing in halotolerance

  • Björn Lindman
Physiology and Growth


The initial rate ofd-glucosamine uptake by the non-halotolerant yeastSaccharomyces cerevisiae was approximately halved as the apparent half saturation constant (Km) and the apparent maximum velocity (Vmax) changed from 6.6mm to 16.4mm and from 22 μmol · g−1 · min−1 to 16 μmol · g−1 · min−1, respectively, when the salinity in the medium was increased from zerom to 0.68m NaCl. Corresponding changes in a high affinity transport system in the halotolerant yeastDebaryomyces hansenii were from 1.1mm to 4.6mm and from 3.1 μmol · g−1 · min−1 to 4.5 μmol · g−1 · min−1, implying a practically unchanged transport capacity. In 2.7m NaCl, Km and Vmax in this system were 24.5mm and 1.1 μmol · g−1 · min−1, respectively, representing a marked decrease in transport capability. Nevertheless, the degree of affinity in this extreme salinity must still be regarded as noteworthy. In addition to the high affinity transport system inD. hansenii, a low affinity system, presumably without relevance ind-glucosamine transport, was observed.


Transport System Initial Rate Maximum Velocity Marked Decrease Transport Capacity 
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  1. Adler, L. 1978. Properties of alkaline phosphatase of the halotolerant yeastDebaryomyces hansenii. -Biochim. Biophys. Acta522: 113–121.PubMedGoogle Scholar
  2. Adler, L. andGustafsson, L. 1980. Polyhydric alcohol production and intracellular amino acid pool in relation to halotolerance of the yeastDebaryomyces hansenii. -Arch. Microbiol.124: 123–130.CrossRefGoogle Scholar
  3. Anand, J. C. andBrown, A. D 1968. Growth rate patterns of the so-called osmophilic and non-osmophilic yeasts in solutions of polyethylene glycol. -J. Gen. Microbiol.52: 205–212.Google Scholar
  4. Brown, A. D. 1976. Microbial water stress. -Bacteriol. Rev.40: 803–846.PubMedGoogle Scholar
  5. Brown, A. D. 1978. Compatible solutes and extreme water stress in eukaryotic micro-organisms. -Adv. Microbiol. Physiol.17: 181–242.Google Scholar
  6. Burger, M. andHejmová, L. 1961. Uptake of metabolizable sugars bySaccharomyces cerevisiae. -Folia Microbiol.6: 80–85.Google Scholar
  7. Dixon, W. J. (ed.) 1977. p. 461–483In Biomedical computer programs. (Revised Nov. 1979). -Univ. Cal. Press, Berkeley, Los Angeles and London.Google Scholar
  8. Gustafsson, L. 1979. The ATP pool in relation to the production of glycerol and heat during growth of the halotolerant yeastDebaryomyces hansenii. -Arch. Microbiol.120: 15–23.CrossRefGoogle Scholar
  9. Gustafsson, L. andNorkrans, B. 1976. On the mechanism of salt tolerance. Production of glycerol and heat during growth ofDebaryomyces hansenii. -Arch. Microbiol.110: 177–183.CrossRefPubMedGoogle Scholar
  10. Higgins, J. 1965. Dynamics and control in cellular reactions. p. 13–46.In R. W. Chance et al. (eds). Control of energy metabolism. -Academic Press, New York.Google Scholar
  11. Hofstee, B. H. 1959. Non-inverted versus inverted plots in enzyme kinetics. -Nature (London)184: 1296–1298.Google Scholar
  12. Lindman, B. 1981.d-Glucosamine uptake by yeasts differing in halotolerance. -FEMS Microbiol. Lett.10: 379–382.CrossRefGoogle Scholar
  13. Norkrans, B. 1966a. Studies on marine occurring yeasts: growth related to pH, NaCl concentration and temperature. -Arch. Mikrobiol.54: 374–392.CrossRefGoogle Scholar
  14. Norkrans, B. 1966b. On the occurrence of yeasts in an estuary off the Swedish westcoast. -Svensk Bot. Tidskr.60: 463–482.Google Scholar
  15. Norkrans, B. andKylin, A. 1969. Regulation of the potassium to sodium ratio and the osmotic potential in relation to salt tolerance in yeasts. -J. Bacteriol.100: 836–845.PubMedGoogle Scholar
  16. Rosé, A. H. (ed.) 1976. p. 11–13.In Chemical Microbiology, 3rd ed. -Butterworths, London and Boston.Google Scholar
  17. van Uden, N. 1967a. Transport-limited growth in the chemostat and its competitive inhibition; a theoretical treatment. -Arch. Mikrobiol.58: 145–154.PubMedGoogle Scholar
  18. van Uden, N. 1967b. Transport-limited fermentation and growth ofSaccharomyces cerevisiae and its competitive inhibition. -Arch. Mikrobiol.58: 155–168.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1981

Authors and Affiliations

  • Björn Lindman
    • 1
  1. 1.Department of Marine Microbiology, Botanical InstituteUniversity of GöteborgGöteborgSweden

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