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Antonie van Leeuwenhoek

, Volume 47, Issue 4, pp 297–306 | Cite as

Effect of NaCl on kinetics ofd-glucosamine uptake in yeasts differing in halotolerance

  • Björn Lindman
Physiology and Growth

Abstract

The initial rate ofd-glucosamine uptake by the non-halotolerant yeastSaccharomyces cerevisiae was approximately halved as the apparent half saturation constant (Km) and the apparent maximum velocity (Vmax) changed from 6.6mm to 16.4mm and from 22 μmol · g−1 · min−1 to 16 μmol · g−1 · min−1, respectively, when the salinity in the medium was increased from zerom to 0.68m NaCl. Corresponding changes in a high affinity transport system in the halotolerant yeastDebaryomyces hansenii were from 1.1mm to 4.6mm and from 3.1 μmol · g−1 · min−1 to 4.5 μmol · g−1 · min−1, implying a practically unchanged transport capacity. In 2.7m NaCl, Km and Vmax in this system were 24.5mm and 1.1 μmol · g−1 · min−1, respectively, representing a marked decrease in transport capability. Nevertheless, the degree of affinity in this extreme salinity must still be regarded as noteworthy. In addition to the high affinity transport system inD. hansenii, a low affinity system, presumably without relevance ind-glucosamine transport, was observed.

Keywords

Transport System Initial Rate Maximum Velocity Marked Decrease Transport Capacity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1981

Authors and Affiliations

  • Björn Lindman
    • 1
  1. 1.Department of Marine Microbiology, Botanical InstituteUniversity of GöteborgGöteborgSweden

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