Medical Electron Microscopy

, Volume 27, Issue 3–4, pp 366–368 | Cite as

Electron microscopic radioautographic study on nucleic acid synthesis of perinatal mouse retina

  • Yongli Kong
  • Tetsuji Nagata
Workshop III: Techniques and Applications of Electron Microscopic Radioautography Workshop III-5


The ocular tissues of ddY mice from fetal to postnatal day 14 were labeledin vitro with3H-thymidine or3H-uridine, and analyzed by light and electron microscopic radioautography. The labeling index (L.I.) with3H-thymidine or grain-numberper-cell with3H-uridine was calculated, and the area of cell organelles was measured by image analysis. The L.I. of retinal cells decreased and disappeared in the vitreal portion at postnatal 3 days and in the seleral portion at postnatal 14 days. The average area of cell organelles in both labeled and unlabeled cells decreased from embryonic 9 days to postnatal 14 days. The grain-number-per-retinal cell labeled with3H-uridine increased until postnatal 1 day and then decreased.

Key words

Mouse Retina Radioautography Image analysis Nucleic acid synthesis 


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Copyright information

© The Clinical Electron Microscopy Society of Japan 1994

Authors and Affiliations

  • Yongli Kong
    • 1
  • Tetsuji Nagata
    • 1
  1. 1.Department of Anatomy and Cell BiologyShinshu University School of MedicineMatsumotoJapan

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