Advertisement

Medical Electron Microscopy

, Volume 27, Issue 3–4, pp 366–368 | Cite as

Electron microscopic radioautographic study on nucleic acid synthesis of perinatal mouse retina

  • Yongli Kong
  • Tetsuji Nagata
Workshop III: Techniques and Applications of Electron Microscopic Radioautography Workshop III-5

Abstract

The ocular tissues of ddY mice from fetal to postnatal day 14 were labeledin vitro with3H-thymidine or3H-uridine, and analyzed by light and electron microscopic radioautography. The labeling index (L.I.) with3H-thymidine or grain-numberper-cell with3H-uridine was calculated, and the area of cell organelles was measured by image analysis. The L.I. of retinal cells decreased and disappeared in the vitreal portion at postnatal 3 days and in the seleral portion at postnatal 14 days. The average area of cell organelles in both labeled and unlabeled cells decreased from embryonic 9 days to postnatal 14 days. The grain-number-per-retinal cell labeled with3H-uridine increased until postnatal 1 day and then decreased.

Key words

Mouse Retina Radioautography Image analysis Nucleic acid synthesis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1).
    Blanks, J. C. andBok, D.: An autoradiographic analysis of postnatal cell proliferation in the normal and degenerative mouse retina.J. Comp. Neurol. 174, 317–328 (1977).CrossRefPubMedGoogle Scholar
  2. 2).
    Carter-Dawson, L. D. andLaVail, M. M.: Rods and cones in the mouse retina. II. Autoradiographic analysis of cell generation using tritiated thymidine.J. Comp. Neurol. 188, 263–272 (1979).PubMedGoogle Scholar
  3. 3).
    Yew, D. W.: Autoradiographic study of neonatal rat retina after labelled uridine uptake.Acta Histochem. 64, 1–4 (1979).PubMedGoogle Scholar
  4. 4).
    Nagata, T.: Techniques and application of electron microscopic radioautography in biomedical research.In: Bi-regional Training Course on Electron Microscopy in Biomedical Research and Diagnosis of Human Diseases (Mukherjee, T. M. andSwift, J. G. ed.), p. 73–90, World Health Organization, Adelaide, Australia, 1991.Google Scholar
  5. 5).
    Kong, Y., Usuda, N. andNagata, T.: Radioautographic study on DNA synthesis of the retina and retinal pigment epithelium of developing mouse embryos.Cell. Mol. Biol. 38, 263–272 (1992).PubMedGoogle Scholar
  6. 6).
    Kong, Y.: Electron microscopic radioautographic study on DNA synthesis in perinatal mouse retina.Cell. Mol. Biol. 39, 55–64 (1993).PubMedGoogle Scholar
  7. 7).
    Kong, Y., Usuda, N., Morita, T., Hanai, T. andNagata, T.: Study on RNA synthesis in the retina and retinal pigment epithelium of mice by light microscopic radioautography.Cell. Mol. Biol. 38, 669–678 (1992).PubMedGoogle Scholar

Copyright information

© The Clinical Electron Microscopy Society of Japan 1994

Authors and Affiliations

  • Yongli Kong
    • 1
  • Tetsuji Nagata
    • 1
  1. 1.Department of Anatomy and Cell BiologyShinshu University School of MedicineMatsumotoJapan

Personalised recommendations