Abstract
TheMaackia amurensis leukoagglutinin (MAL),Sambucus nigra L.I (SNA I), andAmaranthus caudatus (amaranthin; ACL) lectins were used for the study of glycoconjugate residues on normal colonic mucosa and adenocarcinoma by the lectin gold technique. MAL labeled normal colonic mucosa but SNA I did not. ACL stained normal mucosa with regional differences. In contrast, all three lectins labeled most adenocarcinomas. The labeling pattern of MAL was variable but that of SNA I and ACL bound the apical surface of carcinoma cells. Some cells were labeled by SNA I but not by MAL, suggesting thatde novo expression of an α2, 6 sialytrans-ferase was accompanied by malignant transformation. Pretreatment of the sections with galactose oxidase-Schiff sequence abolished the ACL labeling, indicating colonic mucosa and carcinoma cells exhibiting a cryptic form of the T antigen.
Similar content being viewed by others
References
Morgenthaler, J., Kemmner, W. andBrossmer, R.: Sialic acid dependent cell adhesion to collagen IV correlates within vivo tumorigenicity of the human colon carcinoma sublines HCT116, HCT116a and HCT116b.Biochem. Biophys. Res. Commun. 171, 860–866 (1990).
Gong, E-C., Hirohashi, S., Shimosato, Y., Watanabe, M., Ino, Y., Teshima, A. andKodaira, S.: Expression of carbohydrate antigen 19–9 and stage-specific embryonic antigen 1 in nontumorous and tumorous epithelia on the human colon and rectum.J. Natl. Cancer. Inst. 75, 447–454 (1985).
Wolf, B.C., Salem, R.R., Sears, H.F., Horst, D.A., Lavin, P.T., Herlyn, M., Itzkowitz, S.H., Schlom, J. andSteele G.D. Jr.: The expression of colorectal carcinoma-associated antigens in the normal colonic mucosa.Am. J. Pathol. 135, 111–119 (1989).
Itzkowitz, S.H., Yuan, M., Montgomery, C.K., Kjeldsen, T., Takahashi, H.K., Bigbee, W.L. andKim, Y.S.: Expression of Tn, sialosyl-Tn and T antigens in human colon cancer.Cancer Res. 49, 197–204 (1989).
Φrntoft, T.F., Harving, N. andLangkide, N.C.: O-linked mucin type glycoproteins in normal and malignant colon mucosa: lack of T-antigen expression and accumulation of Tn and sialosyl-Tn antigens in carcinoma.Int. J. Cancer,45, 666–672 (1990).
Wang, W.C. andCummings, R.D.: The immobilized leukoagglutinin from the seeds ofMaackia amurensis binds with high affinity to complex-type asn-linked oligosaccharides containing terminal sialic acid-linked α-2,3 to penultimate galactose residues.J. Biol. Chem. 263, 4576–4585 (1988).
Shibuya, N., Goldstein, I.J., Broekaert, W.F., Nsimba-Lubaki, M., Peters, B. andPeumans, W.J.: The elderberry (Sambucus nigra L.) bark lectin recognizes the Neu5Ac (α2,6)-Gal/GalNAc sequence.J. Biol. Chem. 262, 1596–1601 (1987).
Sata, T., Lackie, P.M., Taatjes, D.J., Peumans, W. andRoth, J.. Detection of the Neu5Ac (α2,3)Gal(β1,4)GlcNAc sequence with the leukoagglutinin fromMaackia amurensis; light and electron microscopic demonstration of differential tissue expression of terminal sialic acid in α2, 3 and α2, 6-linkage.J. Histochem. Cytochem. 37, 1577–1588 (1989).
Rinderle, S.J., Goldstein, I.J., Matta, K.L. andRatcliffe, R.M.: Isolation and characterization ofamaranthin, a lectin fromAmaranthin caudatus seeds.Biochemistry,26, 16123–16131 (1990).
Sata, T., Zuber, C., Rinderle, S.J., Goldstein, I.J. andRoth, J.: Expression patterns of the T antigen and the cryptic T antigen in rat fetuses; detection with the lectinAmaranthin.J. Histochem. Cytochem. 38, 763–774 (1990).
Sata, T., Zuber, C. andRoth, J.: Lectin-digoxigenin conjugates: a new hapten system for glycoconjugate cytochemistry.Histochemistry,94, 1–11 (1990).
Sata, T., Roth, J., Zuber, C., Stamm, B. andHeitz, Ph.U.: Expression of α2, 6-linked sialic acid residues in neoplastic but not in normal human colonic mucosa.Am. J. Pathol. 139, 1435–1448 (1991).
Sata, T., Roth, J., Zuber, C., Stamm, B., Rinderle, S.J., Goldstein, I.J. andHeitz, P.U.: Studies on the Thomsen-Fredenreich antigen in human colon with the lectinAmaranthin; normal and neoplastic epithelium express only cryptic T antigen.Lab. Invest. 66, 175–186 (1992).
Cooper, H.S.: Peanut lectin-binding sites in large bowel carcinoma.Lab. Invest. 47, 383–390 (1982).
Cooper, H.S. andReuter, V.E.: Peanut lectin-binding sites in polyps of the colon and rectum. Adenomas, hyperplastic polyps, and adenomas within situ carcinoma.Lab. Invest. 49, 655–661 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sata, T., Roth, J. Lectin histochemistry for the study of glycoconjugate residues on colonic adenocarcinoma. Med Electron Microsc 27, 308–311 (1994). https://doi.org/10.1007/BF02349682
Issue Date:
DOI: https://doi.org/10.1007/BF02349682