Medical Electron Microscopy

, Volume 27, Issue 3–4, pp 297–298 | Cite as

Effects of porcine pancreatic elastase-1 on elastin in human trabecular meshwork

Combined studies of enzymatic digestion and immunoelectron microscopy
  • Takuo Hoya
Symposium III: Fine Structure of the Aqueous Outflow Pathway Symposium III-6
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Abstract

Human trabecular meshwork tissue was embedded in Lowicryl K4M and subjected to electron microscopy. Elastin in the trabecular meshwork was stained using protein A-gold immunostaining method and observed under an electron microscope. For the enzymatic digestion study, the tissue specimens were incubated in various concentrations of solutions of both porcine pancreatic elastase-1 (PPE) and {ie297-1} complex ({ie297-2}), before immunostaining. Many gold particles showing the presence of elastin were found within elastic fibers of the trabecular meshwork. The number of gold particles in the elastic fibers significantly decreased after the enzymatic digestion.

Key words

Human trabecular meshwork Protein A-gold Elastin Porcine pancreatic elastase-1 {ie297-3} complex 

References

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    Gong, H., Trinkaus-Randall, V. andFreddo, T.F.: Ultrastructural immunocytochemical localization of elastin in normal human trabecular meshwork.Curr. Eye Res. 8, 1071–1082 (1989).PubMedGoogle Scholar
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    Tsujii, T., Katayama, K., Naito, I. andSeno, S.: The circulatingα 1-antitrypsin-elastase complex attacks the elastic lamina of blood vessels. An immunohistochemical study.Histochemistry,88, 443–451 (1988).PubMedGoogle Scholar

Copyright information

© The Clinical Electron Microscopy Society of Japan 1994

Authors and Affiliations

  • Takuo Hoya
    • 1
  1. 1.Department of OphthalmologyShinshu University School of MedicineMatsumotoJapan

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