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Medical Electron Microscopy

, Volume 27, Issue 3–4, pp 213–216 | Cite as

Application of Immunoelectron Microscopy to Clinical Medicine

  • Keiichi Watanabe
Educational Lecture
  • 40 Downloads

Abstract

The principles of two representative methods, pre-embedding and post-embedding methods, of immunoelectron microscopy were explained, and their merits and demerits in application for clinical medicine were described. Some examples of the preembedding method were demonstrated with the immunoelectron micrographs. The usefulness of Laser Scanning Microscope in visualizing subcellular localizations of antigens was also discussed.

Key words

Pre-embedding and post-embedding methods Immunoelectron microscopy Laser scanning microscope Subcellular localizations of antigens 

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References

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    Mitani, F., Shimizu, T., Ueno, R., Ishizuka, Y., Izumi, S., Komatsu, N. andWatanabe, K.: Cytochrome P-450 11β and P-450 ssc in adrenal cortex: zonal distribution and intramitochondrial localization by horseradish peroxidase-labeled antibody method.J. Histochem. Cytochem. 30, 1066–1074 (1982).PubMedGoogle Scholar
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    Utsunomiya, H., Komatsu, N., Yoshimura, S., Tsutsumi, Y. andWatanabe, K.: Exact ultrastructural localization of glutathione peroxidase in normal rat hepatocyte: advantage of microwave fixation.J. Histochem. Cytochem. 39, 1167–1174 (1991).PubMedGoogle Scholar

Copyright information

© The Clinical Electron Microscopy Society of Japan 1994

Authors and Affiliations

  • Keiichi Watanabe
    • 1
  1. 1.Department of PathologyTokai University School of MedicineIseharaJapan

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